Erythropoietin 蛋白, Cynomolgus (HEK293, His)

Erythropoietin Protein is a glycoprotein cytokine secreted mainly by the kidneys in response to cellular hypoxia. It regulates erythrocyte growth and balance. EPO binding to its receptor (EPOR) triggers EPOR dimerization, activating JAK2. This initiates downstream events involving effectors like STAT1 and STAT3, crucial for erythrocyte proliferation, differentiation, and maintaining optimal blood cell levels. Erythropoietin Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Erythropoietin protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

促红细胞生成素 (EPO) 是一种糖蛋白细胞因子，主要由肾脏响应细胞缺氧而分泌。EPO在调节红细胞的生长和成熟以及维持体内循环红细胞的适当平衡方面发挥着至关重要的作用。当 EPO 与其受体 (EPOR) 结合时，会触发 EPOR 二聚化，进而激活 JAK2，启动涉及特定下游效应器 (例如 STAT1 和 STAT3) 的一系列级联事件。这些分子途径对于确保红细胞的适当增殖和分化以及维持循环中红细胞的最佳水平至关重要。此外，EPO除了刺激红细胞生成之外，还具有一系列作用，包括血管收缩依赖性高血压、刺激血管生成以及通过激活 EPO 受体促进细胞存活，从而对缺血组织产生抗凋亡作用^[1]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells and the ED₅₀ is typically 1-5 ng/mL.

Cynomolgus

HEK293

C-His;C-10*His

P07865/XP_005549312.1 (A28-R192)

101925524  [NCBI]

APPRLICDSRVLERYLLEAKEAENVTMGCSESCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQAVLANSSQPFEPLQLHMDKAISGLRSITTLLRALGAQEAISLPDAASAAPLRTITADTFCKLFRVYSNFLRGKLKLYTGEACRRGDR

Approximately 19.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.