Erythropoietin receptor/EpoR 蛋白, Mouse (HEK293, His)

Erythropoietin receptor (EpoR) mediates erythropoietin-induced erythroblast proliferation and differentiation. After EPO stimulation, EpoR dimerizes, initiates the JAK2/STAT5 signaling cascade, and can activate STAT1, STAT3, and LYN tyrosine kinases in certain cell types. Erythropoietin receptor/EpoR Protein, Mouse (HEK293, His) is the recombinant mouse-derived Erythropoietin receptor/EpoR protein, expressed by HEK293 , with C-His labeled tag. The total length of Erythropoietin receptor/EpoR Protein, Mouse (HEK293, His) is 225 a.a., with molecular weight of 28-35 kDa.

促红细胞生成素受体 (EpoR) 是促红细胞生成素的关键受体，在 EPO 刺激下协调成红细胞增殖和分化。通过 EpoR 二聚化，它启动 JAK2/STAT5 信号级联，导致各种细胞反应。除了激活特定细胞类型中的 STAT1 和 STAT3 之外，EpoR 还可能参与 LYN 酪氨酸激酶。EPO 结合后，EpoR 形成同二聚体并经历酪氨酸磷酸化，促进与多种含 SH2 结构域的蛋白质（如 LYN、APS、PTPN6、PTPN11、JAK2、PI3 激酶、STAT5A/B、SOCS3、CRKL 和 ATXN2L）相互作用。这些复杂且多方面的相互作用调节下游信号传导事件，包括有丝分裂途径和细胞表面表达。值得注意的是，EpoR 与 NOSIP 和泛素连接酶 NOSIP 的相互作用与 EPO 诱导的细胞增殖有关，凸显了 EpoR 介导的信号传导的调节复杂性。此外，EpoR 与 FSFFV gp55 形成异源寡聚物，并与 INPP5D/SHIP1 结合，进一步扩展其在细胞过程中的功能库。

Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 17.15 ng/mL in the presence of 16 ng/mL of rmEpo, corresponding to a specific activity is 5.831×10⁴ units/mg.

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  Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 17.15 ng/mL in the presence of 16 ng/mL of rmEpo, corresponding to a specific activity is 5.831×10⁴ units/mg.

Mouse

HEK293

C-His

P14753 (A25-P249)

13857  [NCBI]

APSPSLPDPKFESKAALLASRGSEELLCFTQRLEDLVCFWEEAASSGMDFNYSFSYQLEGESRKSCSLHQAPTVRGSVRFWCSLPTADTSSFVPLELQVTEASGSPRYHRIIHINEVVLLDAPAGLLARRAEEGSHVVLRWLPPPGAPMTTHIRYEVDVSAGNRAGGTQRVEVLEGRTECVLSNLRGGTRYTFAVRARMAEPSFSGFWSAWSEPASLLTASDLDP

28-35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.