6Ckine/CCL21B 蛋白, Mouse

6Ckine/CCL21B Protein, Mouse is a homeostatic lymphoid chemokine that contributes to the entry of T cells and dendritic cells into the lymphoid T-zone. It acts through chemokine receptors CCR7 and CXCR3 to promote fibrogenic and inflammatory cytokine production. Exodus-2/CCL21 Protein, Mouse  is a recombinant mouse 6Ckine/CCL21B (S24-G133) expressed by E. coli^[1].

CCL21，也称为 exodus-2 和次级淋巴趋化因子 (SLC)，是属于 CC 趋化因子家族的一种小细胞因子，位于人类基因组中的第 9 号染色体上。它与糖胺聚糖 (GAG) 结合并固定在内皮细胞的表面。作为一种趋化因子，CCL21 抑制造血并刺激趋化性，并且在体外对胸腺细胞和活化的 T 细胞具有趋化性，但对 B 细胞、巨噬细胞或中性粒细胞没有趋化性。同时，CCL21是T细胞迁移和粘附的强效刺激剂，与T细胞上的糖蛋白PSGL-1结合，促进T细胞迁移至次级淋巴器官。CCL21 可通过趋化因子受体 CCR7 和 CXCR3 发挥作用。其中，CCR7是一种GPCR，通常由T细胞亚群中央记忆细胞、胸腺T细胞、B细胞、成熟DC和其他稀有细胞亚群表达。ccl21 可作为中枢神经系统中的小胶质细胞激活剂，仅在濒危或机械损伤的神经元中表达^[1][2]。

CCL21 (intrathecal administration, 0.06 μg/mouse, once, 14 days) results in a significant and instantaneous decrease in paw withdrawal threshold (PWT) to 0.93 g, which returns to the control value of 1.44 g within 48 hours in wild-type C57BL/6 mice, also causes a rapid decrease in PWT to 0.721 g and does not show any recovery throughout the experiment in paucity of lymphoid T cells (plt) mutation mice^[2].
CCL21 (antibodies neutralization 10 μg/injection, twice weekly) reduces Pancreatic ductal adenocarcinoma (PDAC)-induced abdominal hypersensitivity, alleviates pain associated with pancreatic ductal adenocarcinoma and improves health status in situ mouse model of PDAC in C57BL/6 mice injected with K8484 cells^[3].

CCL21(0-3 nM, 24 h) induces a rapid and significant upregulation of P2X4 protein expression of  microglia at concentrations as low as 1 nM^[2].

1.Full biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/mL.
2.Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.The ED₅₀ for this effect is 13.57 ng/mL, corresponding to a specific activity is 7.37×10⁴ U/mg.

Mouse

E. coli

Tag Free

P86792 (S24-G133)

65956

SDGGGQDCCLKYSQKKIPYSIVRGYRKQEPSLGCPIPAILFLPRKHSKPELCANPEEGWVQNLMRRLDQPPAPGKQSPGCRKNRGTSKSGKKGKGSKGCKRTEQTQPSRG

Approximately 12.1-17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4-8.0, 150 mM NaCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Balsam Rizeq, et al. The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers (Basel). 2020 Apr 23;12(4):1036.  [Content Brief]

  [2]. Förster R, et al. CCR7 and its ligands: balancing immunity and tolerance. Nat Rev Immunol. 2008 May;8(5):362-71.  [Content Brief]

  [3]. Knut Biber, et al. Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development. EMBO J. 2011 May 4;30(9):1864-73.  [Content Brief]

  [4]. Tanabe, S. et al. Identification of a new mouse beta-chemokine, thy-mus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells. J Immunol 1997. 159:5671.

  [5]. Michael Hirth, et al. CXCL10 and CCL21 Promote Migration of Pancreatic Cancer Cells Toward Sensory Neurons and Neural Remodeling in Tumors in Mice, Associated With Pain in Patients. Gastroenterology. 2020 Aug;159(2):665-681.e13.  [Content Brief]

  [6]. Alt C, et al. Functional expression of the lymphoid chemokines CCL19 (ELC) and CCL 21 (SLC) at the blood-brain barrier suggests their involvement in G-protein-dependent lymphocyte recruitment into the central nervous system during experimental autoimmune encephalomyelitis. Eur J Immunol. 2002 Aug;32(8):2133-44.  [Content Brief]