Fc gamma RIII/CD16 蛋白, Cynomolgus (HEK293, His)

The FcγRIIIA/CD16a protein is a receptor for IgG and is optimally activated upon binding of aggregated antigen-IgG complexes, inducing antibody-dependent cellular cytotoxicity (ADCC). It mediates IgG effector function on natural killer cells, limiting viral load. Fc gamma RIII/CD16 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Fc gamma RIII/CD16 protein, expressed by HEK293 , with C-His labeled tag. The total length of Fc gamma RIII/CD16 Protein, Cynomolgus (HEK293, His) is 192 a.a., with molecular weight of 33-40 kDa.

FcγRIIIA/CD16a 蛋白作为免疫球蛋白 γ (IgG) 恒定 Fc 片段的受体，在与细胞表面展示的聚集抗原-IgG 复合物结合时最佳激活，从而产生抗体依赖性细胞毒性 (ADCC)。它介导自然杀伤 (NK) 细胞上的 IgG 效应器功能，触发细胞因子的产生、脱颗粒并限制感染期间的病毒载量。此外，FcγRIIIA 参与记忆样适应性 NK 细胞的生成，可通过 ADCC 有效消除病毒感染的细胞并调节 NK 细胞的存活和增殖。作为 Fc 结合亚基，它与 CD247 和/或 FCER1G 接头结合形成功能性信号传导复合物，启动细胞内信号传导途径，例如磷脂酰肌醇 3-激酶信号传导和钙流，这对 NK 细胞激活至关重要。FcγRIIIA 还共刺激 NK 细胞，独立于 IgG 结合触发靶细胞裂解。它在介导治疗性抗体的抗肿瘤活性中发挥作用，并且在连接到单核细胞上后，诱导 IgG 包被的肿瘤细胞发生肿瘤坏死因子-α (TNFA) 依赖性 ADCC。此外，FcγRIIIA 与 CD2 相互作用，有助于 NK 细胞活化和细胞毒性，并与 S100A4 相互作用，抑制 FCGR3A 的 PKC 依赖性磷酸化。

1. Measured by its binding ability in a SPR assay (Biacore T200). Rituximab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIII with an affinity constant of 0.251 μM.
2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD16 at 10 μg/mL (100 μL/well) can bind Biotinylated Human lgG1. The ED₅₀ for this effect is 0.276 μg/mL, corresponding to a specific activity is 3.62×10^3 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD16 at 10 μg/mL (100 μL/well) can bind Biotinylated Human lgG1. The ED₅₀ for this effect is 0.276 μg/mL, corresponding to a specific activity is 3.62×10³ Unit/mg.

Cynomolgus

HEK293

C-His

Q8SPW2 (G17-Q208)

102140945  [NCBI]

GMRAEDLPKAVVFLEPQWYRVLEKDRVTLKCQGAYSPEDNSTRWFHNESLISSQTSSYFIAAARVNNSGEYRCQTSLSTLSDPVQLEVHIGWLLLQAPRWVFKEEESIHLRCHSWKNTLLHKVTYLQNGKGRKYFHQNSDFYIPKATLKDSGSYFCRGLIGSKNVSSETVNITITQDLAVSSISSFFPPGYQ

33-40 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.