Fc gamma RIII/CD16 蛋白, Rhesus Macaque (His)

The Fc gamma RIII/CD16 protein is a receptor for IgG and is optimally activated upon binding to aggregated antigen-IgG complexes, initiating antibody-dependent cellular cytotoxicity (ADCC) and preventing inappropriate effector cell activation. It mediates IgG effector functions on NK cells, generating memory-like adaptive NK cells to efficiently eliminate virally infected cells. Fc gamma RIII/CD16 Protein, Rhesus Macaque (His) is the recombinant Rhesus Macaque-derived Fc gamma RIII/CD16 protein, expressed by HEK293 , with C-His labeled tag. The total length of Fc gamma RIII/CD16 Protein, Rhesus Macaque (His) is 190 a.a., with molecular weight of ~32-41 KDa.

Fc gamma RIII/CD16 蛋白作为免疫球蛋白 γ (IgG) 不变 Fc 片段的受体，并在与细胞表面展示的聚集的抗原-IgG 复合物结合时被最佳激活，启动抗体依赖性细胞毒性 (ADCC) 过程，从而导致裂解抗体包被的细胞。它不结合游离单体 IgG，从而避免在没有抗原触发的情况下不适当的效应细胞激活。该受体介导自然杀伤 (NK) 细胞上的 IgG 效应器功能，结合感染过程中产生的抗原-IgG 复合物，触发 NK 细胞依赖性细胞因子的产生和脱颗粒，从而限制病毒载量和传播。Fc gamma RIII/CD16 对于生成能够产生大量 IFNG 的记忆样适应性 NK 细胞、通过 ADCC 有效消除病毒感染的细胞以及通过防止 NK 细胞祖细胞凋亡来调节 NK 细胞存活和增殖至关重要。作为 Fc 结合亚基，它与 CD247 和/或 FCER1G 接头结合形成功能性信号传导复合物，启动驱动 NK 细胞激活的细胞内信号传导级联。ITAM 依赖性信号传导与 PKC 的受体磷酸化相结合，介导强大的细胞内钙流，导致促炎细胞因子的产生。此外，Fc gamma RIII/CD16 共刺激 NK 细胞并触发靶细胞裂解，不依赖于 IgG 结合，从而介导治疗抗体的抗肿瘤活性。它还在响应无岩藻糖基化 IgG 的增强 ADCC 中发挥作用。该蛋白与含有ITAM的信号亚基形成异寡聚复合物，包括CD247或FCER1G的同二聚体，或CD247和FCER1G的异二聚体，构成功能性受体复合物。Fc gamma RIII/CD16 与抗原复合 IgG 的 Fc 区相互作用，显示出对 IgG1 和 IgG3 同种型的偏好。它进一步与 CD2 相互作用，促进 NK 细胞活化和细胞毒性，并与 S100A4 相互作用，抑制 FCGR3A 的 PKC 依赖性磷酸化。

Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD16 at 10 μg/mL (100 μL/well) can bind Biotinylated Human lgG. The ED₅₀ for this effect is 0.2155 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD16 at 10 μg/mL (100 μL/well) can bind Biotinylated Human lgG. The ED₅₀ for this effect is 0.2155 μg/mL.

Rhesus Macaque

HEK293

C-His

A3RFZ7 (G17-G206)

720006  [NCBI]

GMRAEDLPKAVVFLEPQWYRVLEKDSVTLKCQGAYSPEDNSTRWFHNESLISSQTSSYFIAAARVNNSGEYRCQTSLSTLSDPVQLEVHIGWLLLQAPRWVFKEEESIHLRCHSWKNTLLHKVTYLQNGKGRKYFHQNSDFYIPKATLKDSGSYFCRGLIGSKNVSSETVNITITQDLAVSSISSFFPPG

Approximately 32-41 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.