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  3. FGA 蛋白, Human (His)

FGA 蛋白, Human (His)

目录号: HY-P72194
COA 产品使用指南

FGA 蛋白是一种异六聚体,具有复杂的结构,其中二硫键连接的 α、β 和 γ 链形成两个不同的组。异源三聚体单元采用头对头构象,在 N 末端汇聚成一个紧凑的中心结构域。这种独特的排列突出了 FGA 的复杂组织,强调了其生物学功能所必需的组成亚基之间的相互作用。FGA 蛋白, Human (His) 是重组的 FGA 蛋白,由 E. coli 表达,带有 N-6*His 标签。FGA 蛋白, Human (His) 全长 183 个氨基酸,分子量约为 ~27 kDa。

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规格 价格 是否有货 数量
20 μg ¥1600 In-stock
50 μg ¥2800 In-stock
100 μg ¥4500 询价
> 100 μg   询价  

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描述

FGA Protein, a heterohexamer, features a complex structure with disulfide-linked alpha, beta, and gamma chains forming two distinct sets. The heterotrimeric units adopt a head-to-head conformation, converging at the N-termini in a compact central domain. This unique arrangement highlights the intricate organization of FGA, emphasizing the interplay between its constituent subunits essential for its biological functions. FGA Protein, Human (His) is the recombinant human-derived FGA protein, expressed by E. coli , with N-6*His labeled tag. The total length of FGA Protein, Human (His) is 183 a.a., with molecular weight of ~27 kDa.

研究背景

FGA 蛋白以异六聚体形式存在,通过二硫键形成复杂的结构,包含两组不同的三个不相同的链:α、β 和 γ。异源三聚体单元以头对头的构象排列,N 末端汇聚在一个紧凑的中央结构域中。这种独特的排列强调了 FGA 的复杂组织,展示了其复杂的结构及其组成亚基之间的相互作用,这对于其在生物系统中的功能作用至关重要。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q8WW76 (G36-S218)

基因 ID

/

蛋白结构
N-term
6*His
FGA (G36-S218)
Accession # Q8WW76
C-term
同用名
Fibrinopeptide A; FGA
氨基酸序列

GPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS

分子量

Approximately 27 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

FGA Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGA Protein, Human (His)
目录号:
HY-P72194
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