FGF-12 蛋白, Human (181a.a, His)

FGF-12, vital for nervous system development, positively regulates voltage-gated sodium channels, particularly SCN8A, enhancing neuronal excitability. It achieves this by elevating the voltage dependence of SCN8A fast inactivation. FGF-12 interacts specifically with the C-terminal region of SCN9A. FGF-12 Protein, Human (181a.a, His) is the recombinant human-derived FGF-12 protein, expressed by E. coli , with N-6*His labeled tag. The total length of FGF-12 Protein, Human (181a.a, His) is 181 a.a., with molecular weight of ~23 kDa.

FGF-12 是神经系统发育和功能的关键参与者，通过积极调节电压门控钠通道的活性来发挥其影响。具体而言，FGF-12 通过调节 SCN8A 快速失活的电压依赖性，有助于增强神经元兴奋性，从而影响钠通道行为的动态。这种复杂的调节作用强调了 FGF-12 在塑造神经元活动中的重要性，并强调了它与 SCN9A C 末端区域的相互作用，强调了它参与与电压门控钠通道功能相关的复杂分子相互作用。

Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells.The ED₅₀ for this effect is 1.702 ng/mL, corresponding to a specific activity is 5.875×10⁵ units/mg.

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  Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells.The ED₅₀ for this effect is 1.702 ng/mL, corresponding to a specific activity is 5.875×10⁵ units/mg.

Human

E. coli

N-6*His

P61328-2 (M1-T181)

2257  [NCBI]

MESKEPQLKGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDVFTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYREPSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDST

Approximately 23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.