FGF-9 蛋白, Human (HEK293, N-hFc)

Activin AB protein is a member of the transforming growth factor-β (TGF-β) superfamily and plays a key role in various biological processes such as cell differentiation, proliferation, and apoptosis. It is involved in embryonic development, tissue repair and immune regulation. FGF-9 Protein, Human (HEK293, N-hFc) is the recombinant human-derived FGF-9 protein, expressed by HEK293 , with N-hFc labeled tag.

FGF-9蛋白属于成纤维细胞生长因子（FGF）家族，具有广泛的有丝分裂和细胞存活活性，参与胚胎发育、细胞生长、形态发生、组织修复、肿瘤生长和侵袭等多种生物过程。这种蛋白质最初被认为是一种对培养的神经胶质细胞具有生长刺激作用的分泌因子，主要由神经系统中的神经元产生，这表明它在神经胶质细胞发育中的重要性。FGF-9 的小鼠同源物的表达取决于 Sonic Hedgehog (Shh) 信号传导，小鼠中 FGF-9 的缺失会导致雄性到雌性的性别逆转表型，表明其在睾丸胚胎发生中的作用。值得注意的是 FGF-9 的偏向表达，在肾脏 (RPKM 6.4)、肾上腺 (RPKM 2.1) 和 10 个其他组织中检测到水平升高，强调了其在多个器官的各种生理环境中的潜在重要性。

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is ≤4.765 ng/mL, corresponding to a specific activity is ≥2.099×10⁵ units/mg.

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  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 3.362 ng/mL, corresponding to a specific activity is 2.974×10⁵ units/mg.

Human

HEK293

N-hFc

NP_002001.1 (L4-S208)

2254  [NCBI]

LGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

Approximately 54-57kDa due to the glycosylation.

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  Greater than 95%, as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.