FGFR-4 蛋白, Human (HEK293, Fc)

The leptin R/LEPR protein is a receptor protein that binds to the hormone leptin. It plays a vital role in regulating energy balance and body weight. FGFR-4 Protein, Human (HEK293, Fc) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FGFR-4 Protein, Human (HEK293, Fc) is 348 a.a., with molecular weight of 95-112 kDa.

FGFR-4蛋白作为酪氨酸激酶和成纤维细胞生长因子的细胞表面受体，在调节多种途径中发挥关键作用，包括细胞增殖、分化、迁移、脂质代谢、胆汁酸生物合成、维生素D代谢、葡萄糖摄取、和磷酸盐稳态。在结构上，该蛋白质包含具有三个免疫球蛋白样结构域的胞外区域、单个疏水性跨膜片段和胞质酪氨酸激酶结构域。FGFR-4 通过其细胞外部分与成纤维细胞生长因子相互作用，启动下游信号级联反应，最终影响有丝分裂和分化。该基因表现出广泛的表达，在肺 (RPKM 16.7)、肾 (RPKM 12.5) 和 15 个其他组织中观察到表达水平升高，强调了其在各个器官的不同生理环境中的潜在重要性。

1. Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts and the ED₅₀ is typically 4-25 ng/mL.
2. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 2.694 ng/mL in the presence of 1ng/mL FGF-1, corresponding to a specific activity is 3.71×10⁵ units/mg.

• 
  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 2.694 ng/ml in the presence of 1ng/mL FGF-1, corresponding to a specific activity is 3.71×10⁵ units/mg.

Human

HEK293

C-hFc

NP_002002.3 (L22-D369)

2264  [NCBI]

LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD

95-112 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.