FGFR-4 蛋白, Rhesus Macaque (HEK293, His)

The FGFR-4 protein is a cell surface receptor for tyrosine kinase and fibroblast growth factor. FGFR4, a receptor for FGF-1 and FGF-3 highly expressed in cancer cells, promotes tumor progression in colon cancer by activating Mek/Erk and MMP-7. FGFR-4 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived FGFR-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-4 Protein, Rhesus Macaque (HEK293, His) is 348 a.a., with molecular weight of ~60-80 KDa.

FGFR-4 蛋白作为酪氨酸激酶和成纤维细胞生长因子的细胞表面受体，在调节多种途径中发挥关键作用，包括细胞增殖、分化、迁移、脂质代谢、胆汁酸生物合成、维生素 D 代谢、葡萄糖摄取和磷酸盐稳态。在结构上，该蛋白包括一个具有三个免疫球蛋白样结构域的细胞外区域，一个疏水膜跨段和一个细胞质酪氨酸激酶结构域。通过其与成纤维细胞生长因子相互作用的细胞外部分，FGFR-4 启动下游信号级联，最终影响有丝分裂和分化。该基因表现出广泛的表达，在肺 (RPKM 16.7)、肾 (RPKM 12.5) 和其他 15 个组织中观察到水平升高，强调了其在不同器官的不同生理环境中的潜在意义。FGFR4 是一种在癌细胞中高表达的 FGF-1 和 FGF-3 的受体，通过激活 Mek/Erk 和 MMP-7 促进结肠癌的肿瘤进展。

Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 3.68 ng/mL in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 2.717×10⁵ units/mg.

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  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 3.68 ng/ml in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 2.717×10⁵ units/mg.

Rhesus Macaque

HEK293

C-6*His

XP_001087243.1 (L22-D369)

698494  [NCBI]

LEASEEVELEPCLAPSMEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARASMIVLQNLTLTIDDSLTSSNDDEDPQSHRDSSNGHIYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGIIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDLTWTAATPEARYTD

Approximately 60-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.