Fibronectin 蛋白, Human (HEK293, His)

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. Fibronectin Protein, Human (HEK293, His) is the recombinant human-derived Fibronectin protein, expressed by HEK293 , with C-His labeled tag.

纤连蛋白是一种多功能糖蛋白，通过与胶原蛋白、纤维蛋白、肝素、DNA 和肌动蛋白等多种化合物结合，在细胞相互作用中发挥着至关重要的作用。它参与细胞粘附、运动、调理作用、伤口愈合和细胞形状的维持，强调了它在基本细胞过程中的重要性。纤连蛋白还通过纤连蛋白原纤维形成细胞介导的基质组装过程促进成骨细胞压实，这对成骨细胞矿化至关重要，并调节成骨细胞沉积 I 型胶原。作为 LILRB4 受体的配体，纤连蛋白抑制 FCGR1A/CD64 介导的单核细胞活化。此外，如在名为超纤连蛋白的纤连蛋白聚合物中所观察到的，纤连蛋白诱导原纤维形成的能力赋予增强的粘合性能。值得注意的是，阿纳斯特林和超纤连蛋白均对肿瘤生长、血管生成和转移具有抑制作用，阿纳斯特林激活 p38 MAPK 并抑制溶血磷脂信号传导。纤连蛋白的多方面功能强调了其在细胞动力学中的关键作用及其作为各种病理状况的治疗干预目标的潜力。

1. Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 35%-60% cells will adhere specifically after 35 minutes at 37°C.
2. Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is ≤1.389 μg/mL, corresponding to a specific activity is ≥719.94 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 1.389 μg/ml, corresponding to a specific activity is 719.94 units/mg.

Human

HEK293

C-His

P02751-15 (S607-P1265)

2335  [NCBI]

SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

Approximately 80-96 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.