FKBP14 蛋白, Human (HEK293, His)

The FKBP14 protein is a peptidyl prolyl cis-trans isomerase (PPIase) that uniquely accelerates protein folding, particularly in protein synthesis. FKBP14 particularly favors 4-hydroxyproline-modified substrates, exhibits significant affinity for type III collagen, and has potential effects on type VI and type X collagen. FKBP14 Protein, Human (HEK293, His) is the recombinant human-derived FKBP14 protein, expressed by HEK293 , with C-His labeled tag. The total length of FKBP14 Protein, Human (HEK293, His) is 188 a.a., with molecular weight of ~25 & 27 kDa, respectively.

FKBP14 蛋白作为肽基脯氨酰顺反异构酶 (PPIase) 出现，在加速蛋白质折叠方面发挥独特作用，特别是在蛋白质合成的复杂过程中。值得注意的是，FKBP14 对具有 4-羟脯氨酸修饰的底物表现出特定的偏好，对 III 型胶原蛋白具有明显的亲和力。除了其主要底物之外，FKBP14 还可能将其催化活性扩展到目标 VI 型和 X 型胶原蛋白，这表明对不同胶原蛋白类型的折叠动力学具有更广泛的影响。该蛋白质加速胶原蛋白分子折叠的能力强调了其在调节这些关键细胞外基质成分的结构完整性方面的重要性，强调了 FKBP14 对细胞和组织生理学的潜在影响。需要进一步探索，以阐明 FKBP14 选择性作用于胶原蛋白底物的特定分子机制，并揭示其在蛋白质折叠过程中更广泛的功能含义。

Measured by its ability to convert the substrate, Suc-AAPF-pNA, from Cis to Trans formation. The specific activity is 420.07 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-His

Q9NWM8 (A20-K207)

55033  [NCBI]

ALIPEPEVKIEVLQKPFICHRKTKGGDLMLVHYEGYLEKDGSLFHSTHKHNNGQPIWFTLGILEALKGWDQGLKGMCVGEKRKLIIPPALGYGKEGKGKIPPESTLIFNIDLLEIRNGPRSHESFQEMDLNDDWKLSKDEVKAYLKKEFEKHGAVVNESHHDALVEDIFDKEDEDKDGFISAREFTYK

Approximately 25&27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.