1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. FKBP2 蛋白, Human (HEK293, His)

FKBP2 蛋白, Human (HEK293, His)

目录号: HY-P70333
产品使用指南

FKBP2蛋白在蛋白质折叠的复杂过程中起着核心作用,是一种重要的肽基脯氨酰顺反异构酶(PPIase)。其催化脯氨酸酰亚胺肽键顺反异构化的独特能力可加速动态构象变化,这对于有效蛋白质折叠至关重要。FKBP2 蛋白, Human (HEK293, His) 是重组的 FKBP2 蛋白,由 HEK293 表达,带有 C-6*His 标签。FKBP2 蛋白, Human (HEK293, His) 全长 121 个氨基酸,分子量约为 ~17.0 kDa。

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描述

FKBP2 protein plays a central role in the complex process of protein folding and is an important peptidyl prolyl cis-trans isomerase (PPIase). Its unique ability to catalyze the cis-trans isomerization of proline imide peptide bonds accelerates dynamic conformational changes that are critical for efficient protein folding. FKBP2 Protein, Human (HEK293, His) is the recombinant human-derived FKBP2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FKBP2 Protein, Human (HEK293, His) is 121 a.a., with molecular weight of ~17.0 kDa.

研究背景

FKBP2 蛋白作为复杂的蛋白质折叠过程的关键参与者而占据中心舞台,展示了其作为肽基脯氨酰顺反异构酶 (PPIase) 的重要作用。FKBP2 具有催化寡肽中脯氨酸酰亚胺肽键顺反异构化的独特能力,可积极加速对蛋白质有效折叠至关重要的动态构象变化。这种酶活性强调了 FKBP2 在促进新生或错误折叠多肽的正确成熟和结构完整性方面的重要性,从而有助于细胞蛋白质稳态的整体维持。作为 PPIase 家族的一员,FKBP2 在复杂的蛋白质折叠过程中发挥着重要作用,需要进一步探索以揭示 FKBP2 积极参与这一关键细胞过程的特定分子机制和细胞环境。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P26885 (A22-L142)

基因 ID
蛋白结构
N-term
FKBP2 (A22-L142)
Accession # P26885
6*His
C-term
同用名
rHuPeptidyl-prolyl cis-trans isomerase FKBP2 Gene/FKBP2, His; Peptidyl-prolyl cis-trans isomerase FKBP2(FKBP2 for short); also named 13 kDa FK506-binding protein; FK506-binding protein 2; Immunophilin FKBP13; Rotamase
氨基酸序列

ATGAEGKRKLQIGVKKRVDHCPIKSRKGDVLHMHYTGKLEDGTEFDSSLPQNQPFVFSLGTGQVIKGWDQGLLGMCEGEKRKLVIPSELGYGERGAPPKIPGGATLVFEVELLKIERRTEL

分子量

Approximately 17.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FKBP2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FKBP2 Protein, Human (HEK293, His)
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