1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. FkpA 蛋白, E.coli (His-SUMO)

FkpA 蛋白, E.coli (His-SUMO)

目录号: HY-P71477
产品使用指南

FkpA 蛋白在细胞过程中发挥着核心作用,是复杂的蛋白质折叠过程中的关键催化剂。其催化效率优异,可促进脯氨酸-亚胺肽键的顺反异构化,从而加速蛋白质的整体折叠。FkpA 蛋白, E.coli (His-SUMO) ) 是重组的 FkpA 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。

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描述

The FkpA protein plays a central role in cellular processes, acting as a key catalyst in the complex dance of protein folding. Its catalytic efficiency is excellent in promoting the cis-trans isomerization of proline-imine peptide bonds, thus accelerating the overall folding of proteins. FkpA Protein, E.coli (His-SUMO) ) is the recombinant E. coli-derived FkpA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

研究背景

在细胞机器的最前沿,FkpA 蛋白作为加速复杂的蛋白质折叠过程的关键催化剂而出现。其催化能力在促进寡肽内脯氨酸酰亚胺肽键的顺反异构化方面尤其明显。通过协调这些精确的结构变化,FkpA 显着加速了蛋白质的整体折叠,揭示了其在确保及时、准确地形成功能性蛋白质结构方面的重要作用。这种分子技巧凸显了 FkpA 在细胞过程复杂编排中的重要性,强调了它对维持细胞稳态的贡献。

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

P65764 (A26-K270)

基因 ID

58460171  [NCBI]

蛋白结构
N-term
6*His-SUMO
FkpA (A26-K270)
Accession # P65764
C-term
同用名
fkpA; c4121FKBP-type peptidyl-prolyl cis-trans isomerase FkpA; PPIase; EC 5.2.1.8; Rotamase
氨基酸序列

AEAAKPATTADSKAAFKNDDQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTLQAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEAPKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLVIPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK

分子量

Approximately 42.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FkpA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FkpA Protein, E.coli (His-SUMO)
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