FOLR1 蛋白, Canine (HEK293, His)

FOLR1 is a folic acid receptor that binds to folic acid and reductive folic acid derivatives and promotes the entry of 5-methyltetrahydrofolate and folate analogs into the cell interior. FOLR1 enhances the stability and nuclear translocation of β-catenin through the EGFR/AKT/GSK3β axis, thereby promoting the proliferation and migration of laryngeal squamous cell carcinoma (LSCC). FOLR1 is highly expressed in a variety of tumors and is a potential prognostic and therapeutic target for many cancers. FOLR1 Protein, Canine (HEK293, His) is the recombinant canine-derived FOLR1 protein, expressed by HEK293 , with C-His labeled tag.

FOLR1 是叶酸受体 (FOLR) 家族的成员，FOLR1 蛋白是叶酸摄取的关键介质，与叶酸和还原性叶酸衍生物结合，促进 5-甲基四氢叶酸和叶酸类似物进入细胞内部。FOLR1 对正常胚胎发育和正常细胞增殖也很重要。FRA 的自身抗体与神经发育疾病有关，特别是脑叶酸缺乏症，精神分裂症和自闭症谱系障碍。FOLR1 通过 EGFR/AKT/GSK3β 轴增强 β-catenin 的稳定性和核易位，从而促进喉鳞状细胞癌 (LSCC) 的增殖和迁移。FOLR1 在多种肿瘤中高表达是许多癌症的潜在预后和治疗靶点。

Measured by its binding ability in a functional ELISA. Immobilized FOLR1 at 2 μg/mL can bind Anti-FOLR1 antibody, the ED₅₀ for this effect is 0.9311 ng/mL .

Canine

HEK293

C-6*His

XP_851993.1 (R25-M228)

609612  [NCBI]

RTELLNVCMDAKHHKEKPSPEDGLHKQCSPWKKNSCCFANTSREAHKDISYLYRFNWNHCGSMTPACKKHFIQDTCLYECSPNLGPWIQEVNQSWRKERILHVPLCKEDCEQWWQDCRTSYTCKSNWHKGWNWTSGYNQCPEGAACHPFHFYFPTSAALCSEIWSHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYARAM

Approximately 27-40 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.