Frataxin/FXN 蛋白, Human (His, Myc)

Frataxin/FXN protein activates the core iron-sulfur cluster assembly complex and accelerates sulfur transfer for [2Fe-2S] cluster assembly. It plays a key role in the de novo synthesis of clusters, delivering persulfide to the ISCU. Frataxin/FXN Protein, Human (His, Myc) is the recombinant human-derived Frataxin/FXN protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of Frataxin/FXN Protein, Human (His, Myc) is 210 a.a., with molecular weight of ~33 kDa.

Frataxin/FXN 蛋白作为核心铁硫簇 (ISC) 组装复合物内的激活剂，促进过硫化物转移至支架蛋白 ISCU 并参与 [2Fe-2S] 簇组装。它加速硫从 NFS1 过硫化物中间体转移到 ISCU 和 L-半胱氨酸和谷胱甘肽等小硫醇，从而导致过硫化和硫化物释放。Frataxin/FXN 是 [2Fe-2S] 簇从头合成的组成部分，该簇由产生过硫化物的半胱氨酸脱硫酶复合物 (NFS1:LYRM4:NDUFAB1) 启动，并以 FXN 依赖性方式递送至 ISCU。该复合物由 FDX2 稳定，为 [2Fe-2S] 簇组装提供还原当量。该簇随后从 ISCU 转移到伴侣蛋白，包括 HSCB、HSPA9 和 GLRX5。Frataxin/FXN 可能通过其亚铁氧化酶活性（特别是其寡聚形式）在防止铁催化的氧化应激方面发挥作用。它还可能充当铁伴侣，保护乌头酸酶 [4Fe-4S]2+ 簇并促进酶重新激活。此外，Frataxin/FXN 可能作为 FECH 的高亲和力铁结合伴侣，有助于线粒体血红素生物合成，调节 ACO1 的 RNA 结合活性，并可能影响细胞质铁硫蛋白的生物合成，总体影响氧化应激抵抗力和细胞生存。

Human

E. coli

C-Myc;N-10*His

Q16595 (M1-A210)

2395  [NCBI]

MWTLGRRAVAGLLASPSPAQAQTLTRVPRPAELAPLCGRRGLRTDIDATCTPRRASSNQRGLNQIWNVKKQSVYLMNLRKSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDLSSLAYSGKDA

Approximately 33 kDa

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  Greater than 92% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.