Frizzled-10/CD350 蛋白, Mouse (HEK293, His)

Frizzled-10/CD350 protein is a receptor for Wnt proteins that functions in the canonical Wnt/β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target gene activation. Secondary signaling pathways involve PKC and calcium flux. Frizzled-10/CD350 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Frizzled-10/CD350 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-10/CD350 Protein, Mouse (HEK293, His) is 141 a.a., with molecular weight of 20-24 kDa.

Frizzled-10/CD350 蛋白作为 Wnt 蛋白的受体，主要在经典 Wnt/β-连环蛋白信号通路中发挥作用。该途径协调混乱蛋白质的激活，抑制 GSK-3 激酶，促进 β-连环蛋白的核积累，并触发 Wnt 靶基因的激活。除了经典途径外，一些家族成员还发现了涉及 PKC 和钙通量的次级信号传导途径。鉴于 PKC 在 Wnt 介导的 GSK-3 激酶失活中的明显作用，这些途径及其潜在整合之间的相互作用仍有待充分阐明。Frizzled-10 可能在组织形态发生期间或分化组织内的极性信息转导和传递中发挥至关重要的作用。与 MYOC 和 WNT7B 的相互作用强调了它参与复杂的细胞过程和信号转导级联。

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA. The ED₅₀ for this effect is 38.32 ng/mL.

Mouse

HEK293

C-6*His

Q8BKG4/NP_780493.1(I22-G162)

93897  [NCBI]

ISSMDLERPGDGKCQPVEIPMCKDIGYNTTRMPNLMGHENQREAAIQLHEFAPLVEYGCHSHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFKFRWPDSLDCSKLPNKNDPNYLCMEAPNNGSDEPSRG

Approximately 20-24 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.