Frizzled-4/CD344 蛋白, Human (HEK293, His)

The Frizzled-4/CD344 protein is a receptor for Wnt proteins and is associated with the classical β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. In retinal vascularization, it acts as a receptor for Wnt proteins and Norrin (NDP), stimulating β-catenin accumulation and LEF/TCF-mediated transcription. Frizzled-4/CD344 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-4/CD344 Protein, Human (HEK293, His) is 144 a.a., with molecular weight of ~22-25 kDa.

Frizzled-4/CD344 蛋白作为 Wnt 蛋白的受体，特别与经典 β-连环蛋白信号通路相关，涉及蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后的Wnt 靶基因。在视网膜血管化的背景下，Frizzled-4 通过充当 Wnt 蛋白和诺里蛋白 (NDP) 的受体发挥着关键作用，促进 β-catenin 积累并刺激 LEF/TCF 介导的转录程序。尽管在一些家族成员中观察到以 PKC 和钙通量为特征的次级信号传导途径，但其与经典途径的整合，特别是在 Wnt 介导的 GSK-3 激酶失活的背景下，仍然不确定。通过观察到与 MAGI3、NDP、TSKU 和磷脂酰肌醇蛋白聚糖 GPC3 的相互作用，表明 Frizzled-4 参与组织形态发生和极性信息的细胞间传递，突出了其在细胞过程和信号级联中的复杂作用。

Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED₅₀ for this effect is 26.59 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED₅₀ for this effect is 26.59 ng/mL.

Human

HEK293

C-His

Q9ULV1 (F37-E180)

8322  [NCBI]

FGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEE

Approximately 22-25 kDa due to glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.