Frizzled-5 蛋白, Mouse (CHO, Fc)

Frizzled-5 is a Wnt receptor that activates canonical Wnt/β-catenin signaling through WNT2, WNT10B, and WNT5A. It participates in a potential second pathway involving PKC and calcium flux, affecting Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5 Protein, Mouse (CHO, Fc) is the recombinant mouse-derived Frizzled-5 protein, expressed by CHO , with C-hFc labeled tag.

Frizzled-5 是 Wnt 蛋白的受体，表现出微妙的激活特性，对 WNT2、WNT10B 和 WNT5A 有反应，但在体外对 WNT2B 或 WNT4 没有亲和力。它对经典 Wnt/β-连环蛋白信号传导的参与至关重要，可导致蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后 Wnt 靶基因的激活。此外，第二条信号通路可能涉及 PKC 和钙通量，增加了复杂性，对 Wnt 介导的 GSK-3 激酶失活产生影响。Frizzled-5 参与组织形态发生和潜在参与极性信息的转换，强调了其多功能作用。值得注意的是，在神经元中，WNT7A 激活促进突触形成。它的贡献延伸到卵黄囊血管生成和胎盘血管化。通过不饱和脂肪酸分子的结合促进同二聚化，以及与 WNT2B、WNT7A 和 GOPC 的相互作用进一步强调了 Frizzled-5 在复杂的信号网络中的多方面调节功能。

Mouse

CHO

C-hFc

Q9EQD0 (A27-P167)

14367  [NCBI]

52-56 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.