Frizzled-7 蛋白, Human (HEK293, His)

Frizzled-7 (FZD7) is a Wnt receptor that primarily activates the β-catenin classical pathway, involving Disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Some family members exhibit a second PKC and calcium flux signaling pathway, but the integration is unclear. Frizzled-7/FZD7 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-7/FZD7 protein, expressed by HEK293 , with C-His labeled tag.

Frizzled-7 (FZD7) 是 Wnt 蛋白的受体，主要通过 β-连环蛋白经典信号通路发挥作用，启动涉及蓬乱蛋白、GSK-3 激酶抑制、β-连环蛋白核积累和激活的级联反应。Wnt 靶基因。虽然在一些家族成员中观察到涉及 PKC 和钙通量的第二个信号通路，但其独特性以及与经典通路的潜在整合仍不清楚，PKC 似乎是 Wnt 介导的 GSK-3 激酶失活所必需的。WNT8 的激活会诱导 β-连环蛋白靶基因的表达。配体激活后，FZD7 与 CCDC88C/DAPLE 相互作用，取代 DVL1 并抑制经典 Wnt 信号传导，导致 CCDC88C 激活 G 蛋白并启动非经典 Wnt 反应。这种复杂的参与表明 FZD7 在组织形态发生和/或分化组织中转导极性信息方面的潜在作用。此外，在微生物感染的情况下，FZD7 充当结肠上皮中艰难梭菌毒素 TcdB 的受体。

Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated Wnt-5a at 100 ng/mL and Frizzled-7 dilutions at 0.98-2000 ng/mL. The ED₅₀ for this effect is 73.1 ng/mL.

Human

HEK293

C-6*His

O75084 (Q33-L185)

8324  [NCBI]

QPYHGEKGISVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAYPTAPYL

Approximately 23-33 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.