FUBP1 蛋白, Human (HEK293, C-His)

FUBP1 Protein, a pivotal regulator of MYC expression, binds to the far-upstream element (FUSE) upstream of the MYC promoter, dynamically acting as both an activator and repressor of gene expression. It forms a complex with PUF60, associated with FUSE DNA, implying involvement in intricate molecular processes. Interaction with PUF60 and JTV1 suggests potential cooperative actions in gene expression modulation. FUBP1 Protein, Human (HEK293, C-His) is the recombinant human-derived FUBP1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of FUBP1 Protein, Human (HEK293, C-His) is 644 a.a., with molecular weight of ~95 kDa.

FUBP1 蛋白作为 MYC 表达的关键调节因子，通过与位于 MYC 启动子上游的单链远上游元件 (FUSE) 结合来发挥其控制作用。它在转录调节中的作用是动态的，因为它既可以充当基因表达的激活剂，也可以充当基因表达的阻抑剂。FUBP1 与 PUF60 形成复合物，该复合物与远上游元件 (FUSE) DNA 片段相关，表明其参与复杂的分子过程。此外，FUBP1 与 PUF60 和 JTV1 相互作用，表明在基因表达调节中存在潜在的协同作用。

Human

HEK293

C-10*His

Q96AE4-1 (M1-Q644)

8880

MADYSTVPPPSSGSAGGGGGGGGGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQLQERAGVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDVPIPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ

Approximately 95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol or PBS, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.