1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. fum 蛋白, Corynebacterium glutamicum (His)

fum 蛋白, Corynebacterium glutamicum (His)

目录号: HY-P702122
产品使用指南

Fum 蛋白在三羧酸 (TCA) 循环中至关重要地催化富马酸向 L-苹果酸的相互转化,维持细胞能量代谢中的代谢物流动。如果精确调节这一关键步骤,有助于产生能量并产生对细胞过程至关重要的中间体。fum 蛋白, Corynebacterium glutamicum (His) 是重组的 发酵蛋白,由 E. coli 表达,带有 N-6*His 标签。fum 蛋白, Corynebacterium glutamicum (His) 全长 469 个氨基酸。

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描述

Fum proteins crucially catalyze the interconversion of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, maintaining metabolite flow in cellular energy metabolism. This critical step, if precisely regulated, helps generate energy and generates intermediates critical to cellular processes. fum Protein, Corynebacterium glutamicum (His) is the recombinant fum protein, expressed by E. coli , with N-6*His labeled tag. The total length of fum Protein, Corynebacterium glutamicum (His) is 469 a.a., .

研究背景

fum 蛋白在三羧酸 (TCA) 循环中发挥着至关重要的作用,它催化富马酸向 L-苹果酸的立体特异性相互转化。这种酶活性对于维持 TCA 循环(细胞能量代谢的中心途径)内代谢物的流动至关重要。通过 fum 将富马酸转化为 L-苹果酸是一个关键步骤,有助于产生能量和生成各种细胞过程必需的中间体。这种酶促反应的精确调节凸显了烟气在细胞内协调代谢流和能量产生中的重要性。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

Q8NRN8 (M1-F469)

基因 ID

/

蛋白结构
N-term
6*His
fum (M1-F469)
Accession # Q8NRN8
C-term
同用名
fumC; Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

fum Protein, Corynebacterium glutamicum (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
fum Protein, Corynebacterium glutamicum (His)
目录号:
HY-P702122
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