Fusion glycoprotein F0/F 蛋白, HRSVA (His, B2M)

The fusion glycoprotein F0/F protein is initially an inactive precursor that is cleaved by furin-like proteases to produce the mature F1 and F2 fusion glycoproteins. As a class I viral fusion protein, it undergoes prefusion, prehairpin, and postfusion states. Fusion glycoprotein F0/F Protein, HRSVA (His, B2M) is the recombinant Virus-derived Fusion glycoprotein F0/F protein, expressed by E. coli , with N-B2M, N-6*His labeled tag. The total length of Fusion glycoprotein F0/F Protein, HRSVA (His, B2M) is 503 a.a., with molecular weight of ~69.9 kDa.

融合糖蛋白 F0/F 蛋白作为无活性前体，在两个位点被弗林蛋白酶样蛋白酶裂解，生成成熟的 F1 和 F2 融合糖蛋白。作为I类病毒融合蛋白，它表现出至少三种构象状态：融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和浆细胞膜融合过程中，卷曲螺旋区域采用发夹三聚体结构，将融合肽置于胞外域的 C 末端区域附近。这种结构排列驱动病毒和细胞膜的并置和随后的融合，促进核衣壳递送到细胞质中。重要的是，这种融合与 pH 无关，并且发生在质膜或内体膜上。F1-F2 三聚体（F 蛋白）发挥双重作用，通过与宿主硫酸乙酰肝素结合帮助附着于宿主细胞，并通过与宿主 IGFR1 相互作用促进进入宿主细胞。此外，感染期间在质膜上表达的 F 蛋白可以介导细胞-细胞融合，导致合胞体形成，这是一种可能导致组织坏死的细胞病变效应。此外，F 蛋白可能在感染后期触发 p53 依赖性细胞凋亡。

Virus

E. coli

N-B2M;N-6*His

P03420 (N27-T529)

/

NITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPPTNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGVGSAIASGVAVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMSIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEINLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGMDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLHNVNAGKSTTNIMITT

Approximately 69.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.