G-CSFR/CD114 蛋白, Human (HEK293, His)

The G-CSFR/CD114 Protein, acting as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocytic maturation, playing a pivotal role in the proliferation, differentiation, and survival of neutrophilic lineage cells. Additionally, it may participate in cell surface adhesion or recognition events. The homodimeric receptor binds two CSF3 molecules and interacts with CEACAM1, which down-regulates the CSF3R-STAT3 pathway through the recruitment of PTPN6, leading to the dephosphorylation of CSF3R. G-CSFR/CD114 Protein, Human (HEK293, His) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293, with C-His labeled tag. The total length of G-CSFR/CD114 Protein, Human (HEK293, His) is 603 a.a., with molecular weight of 90-110 kDa.

G-CSFR/CD114 是粒细胞集落刺激因子 (CSF3) 的受体，通过控制中性粒细胞谱系细胞的增殖、分化和存活，在协调粒细胞成熟中发挥着关键作用。这种同二聚体受体与两个 CSF3 分子结合，形成调节重要细胞过程的关键相互作用。除了参与细胞成熟之外，G-CSFR/CD114 还可能有助于细胞表面的粘附或识别事件。此外，它与 CEACAM1 的相互作用充当调节轴，通过招募 PTPN6 下调 CSF3R-STAT3 通路，随后 PTPN6 使 CSF3R 去磷酸化并调节细胞信号传导动力学。

Immobilized Human G-CSF R, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC₅₀ of ≤ 6.0 ng/mL determined by ELISA.

Human

HEK293

C-His

Q99062-1 (E25-H627)

1441  [NCBI]

ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTPEGSELH

90-110 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.