G-CSFR/CD114 蛋白, Human (HEK293)

The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with tag free. The total length of G-CSFR/CD114 Protein, Human (HEK293) is 597 a.a., with molecular weight of ~85.36 kDa.

G-CSFR/CD114 是粒细胞集落刺激因子 (CSF3) 的受体，通过控制中性粒细胞谱系细胞的增殖、分化和存活，在协调粒细胞成熟中发挥着关键作用。这种同二聚体受体与两个 CSF3 分子结合，形成调节重要细胞过程的关键相互作用。除了参与细胞成熟之外，G-CSFR/CD114 还可能有助于细胞表面的粘附或识别事件。此外，它与 CEACAM1 的相互作用充当调节轴，通过招募 PTPN6 下调 CSF3R-STAT3 通路，随后 PTPN6 使 CSF3R 去磷酸化并调节细胞信号传导动力学。

Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED₅₀ for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

• 
  Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED₅₀ for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

Human

HEK293

Tag Free

Q99062/NP_000751.1 (E25-P621)

1441  [NCBI]

ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP

Approximately 85.36 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.