Galectin-3/LGALS3 蛋白, Human (HEK293, His)

The Galectin-3/LGALS3 protein is a galactose-specific lectin known for its diverse roles in cellular processes. It binds IgE and synergizes with α-3 and β-1 integrins to promote CSPG4-induced endothelial cell migration. Galectin-3/LGALS3 Protein, Human (HEK293, His) is the recombinant human-derived Galectin-3/LGALS3 protein, expressed by HEK293 , with C-6*His labeled tag.

Galectin-3/LGALS3 蛋白是一种半乳糖特异性凝集素，以其在细胞过程中的多种作用而闻名。它结合 IgE，并与 α-3、β-1 整合素协同作用，促进 CSPG4 诱导的内皮细胞迁移。与 DMBT1 一起，它对于早期胚胎发生过程中柱状上皮细胞的终末分化至关重要。在细胞核中，该蛋白作为前体 mRNA 剪接因子，积极参与急性炎症反应，影响中性粒细胞活化、粘附、单核细胞和巨噬细胞的化学吸引、凋亡中性粒细胞的调理作用和肥大细胞活化。它与 TRIM16 的合作可以协调识别膜损伤，触发核心自噬调节因子 ATG16L1 和 BECN1 的动员，以应对受损的内膜。该蛋白可能形成同源或异源二聚体，并与各种伙伴结合，包括 DMBT1、CD6、ALCAM、ITGA3、ITGB1、CSPG4、LGALS3BP、LYPD3、ZFTRAF1、UACA、TRIM16 和 TMED10，促进多种细胞相互作用，包括自噬和分泌。

Measured by the ability of the immobilized protein to support the adhesion of TF-1 Human blood leukemia cells. The ED₅₀ this effect is ≤6.301 μg/ml, corresponding to a specific activity is ≥158.705 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of TF-1 Human blood leukemia cells. The ED₅₀ for this effect is 6.301 μg/ml, corresponding to a specific activity is 158.705 units/mg.

Human

HEK293

C-6*His

AAH53667.1 (A2-I250)

3958  [NCBI]

ADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYHGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSAPGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI

Approximately 33-40 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 3 mM DTT, pH 7.4 or PBS, pH 7.4, 3 mM DTT, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.