1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GALNT10 蛋白, Human (HEK293, His)

GALNT10 蛋白, Human (HEK293, His)

目录号: HY-P76354
COA 产品使用指南 技术支持

GALNT10 蛋白在 O-连接寡糖生物合成的启动中发挥着至关重要的作用,促进 N-乙酰基-D-半乳糖胺残基转移到蛋白质受体上的特定丝氨酸或苏氨酸残基。凭借其催化活性,GALNT10 对 Muc5Ac 和 EA2 肽等底物表现出特异性,有助于蛋白质糖基化和随后的细胞功能的复杂过程。GALNT10 蛋白, Human (HEK293, His) 是重组的 GALNT10 蛋白,由 HEK293 表达,带有 N-His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

GALNT10 protein assumes a crucial role in the initiation of O-linked oligosaccharide biosynthesis, facilitating the transfer of an N-acetyl-D-galactosamine residue to specific serine or threonine residues on protein receptors. With its catalytic activity, GALNT10 demonstrates specificity towards substrates such as Muc5Ac and EA2 peptides, contributing to the intricate process of protein glycosylation and subsequent cellular functions. GALNT10 Protein, Human (HEK293, His) is the recombinant human-derived GALNT10 protein, expressed by HEK293 , with N-His labeled tag.

研究背景

GALNT10 是 GALNT(N-乙酰半乳糖胺基转移酶)家族的成员,在 O-连接寡糖生物合成的启动中发挥着关键作用。该酶催化 N-乙酰基-D-半乳糖胺 (GalNAc) 残基转移到蛋白质受体上的丝氨酸或苏氨酸残基,标志着蛋白质糖基化的第一步。GALNT10 对特定底物(例如 Muc5Ac 和 EA2 肽)表现出糖基转移酶活性。通过这个过程,GALNT10 通过添加糖部分来促进蛋白质的多样化和修饰,从而影响各种细胞功能和过程。该酶对某些底物的特异性强调了其在调节生物系统中糖基化模式中的作用。

生物活性

Measured by its ability to transfer GalNAc from UDP-GalNAc to peptide MUC5AC-3/13 that incubate at 37°C for 20 min. The specific activity is 688.65 pmol/min/μg.

种属

Human

表达系统

HEK293

标签

N-10*His

蛋白编号

Q86SR1-1/NP_938080.1 (T39-N603)

基因 ID
蛋白结构
N-term
His
GALNT10 (T39-N603)
Accession # Q86SR1-1/NP_938080.1
C-term
同用名
Polypeptide N-acetylgalactosaminyltransferase 10; GalNAc-T10
氨基酸序列

TPGGSGAAVAPAAGQGSHSRQKKTFFLGDGQKLKDWHDKEAIRRDAQRVGNGEQGRPYPMTDAERVDQAYRENGFNIYVSDKISLNRSLPDIRHPNCNSKRYLETLPNTSIIIPFHNEGWSSLLRTVHSVLNRSPPELVAEIVLVDDFSDREHLKKPLEDYMALFPSVRILRTKKREGLIRTRMLGASVATGDVITFLDSHCEANVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRYETQAGDAMRGAFDWEMYYKRIPIPPELQKADPSDPFESPVMAGGLFAVDRKWFWELGGYDPGLEIWGGEQYEISFKVWMCGGRMEDIPCSRVGHIYRKYVPYKVPAGVSLARNLKRVAEVWMDEYAEYIYQRRPEYRHLSAGDVAVQKKLRSSLNCKSFKWFMTKIAWDLPKFYPPVEPPAAAWGEIRNVGTGLCADTKHGALGSPLRLEGCVRGRGEAAWNNMQVFTFTWREDIRPGDPQHTKKFCFDAISHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVSGSCMDCSESDHRIFMNTCNPSSLTQQWLFEHTNSTVLEKFNRN

分子量

Approximately 70-75 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GALNT10 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GALNT10 Protein, Human (HEK293, His)
目录号:
HY-P76354
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