GCGR 蛋白, Human (HEK293, N-His, C-Myc)

The GCGR protein is a glucagon G protein-coupled receptor that is critical in blood glucose regulation and actively controls hepatic glucose production. It promotes glycogen hydrolysis and gluconeogenesis, which are critical for the fasting response. GCGR Protein, Human (HEK293, N-His, C-Myc) is the recombinant human-derived GCGR protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GCGR Protein, Human (HEK293, N-His, C-Myc) is 111 a.a., with molecular weight of ~35 kDa.

GCGR 蛋白作为胰高血糖素的 G 蛋白偶联受体，在血糖水平和葡萄糖稳态的调节中发挥着关键作用。它通过促进糖原水解和糖异生来积极调节肝葡萄糖的产生，使其成为禁食反应的关键介质。配体结合后，受体会发生构象变化，通过鸟嘌呤核苷酸结合蛋白（G 蛋白）启动信号传导，随后调节下游效应子，例如腺苷酸环化酶。这种调节导致腺苷酸环化酶的激活。此外，该受体通过磷脂酰肌醇-钙第二信使系统参与信号传导，强调了其在葡萄糖调节中的多方面作用。

Measured by its binding ability in a functional ELISA. Immobilized human GCGR at 2 μg/mL can bind Anti-GCGR recombinant antibody, the EC₅₀ is <6.666 ng/mL.

Human

HEK293

C-Myc;N-10*His

P47871 (A26-K136)

2642  [NCBI]

AQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIEVQKEVAK

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.