GFRA1/GDNFR-alpha-1 蛋白, Canine (HEK293, His)

GFRA1 is a co-receptor that recognizes the GDNF ligand family and is involved in the regulation of proliferation, differentiation and migration of neuronal cells. GFRA1 promotes autophagy through the SRC-AMP-activated protein kinase (AMPK) signaling pathway, thereby contributing to the development of cisplatin-induced chemotherapy resistance to osteosarcoma. GFRA1/GDNFR-alpha-1 Protein, Canine (HEK293, His) is the recombinant canine-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag.

糖基磷脂酰肌醇连接的 GDNF (胶质细胞源性神经营养因子) 受体 α (GFRA) 是一种识别 GDNF 配体家族的辅助受体，在神经系统的发育和维持中起着至关重要的作用。GFRA1 参与神经元细胞增殖、分化和迁移的调节。GFRA1 也与癌细胞的进展和转移有关。GFRA1 通过 SRC-AMP 激活的蛋白激酶 (AMPK) 信号通路促进自噬，从而促进顺铂诱导的骨肉瘤化疗耐药的发展。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Canine GDNF at 1 μg/mL binds Recombinant Canine GFR alpha-1/GDNF R alpha-1. The kD is 4.078 nM.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Canine GDNF at 1 μg/mL binds Recombinant Canine GFR alpha ‑1/GDNF R alpha ‑1. The kD is 4.078nM.

Canine

HEK293

C-10*His

XP_852087.1 (D25-S429)

609114  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFLPDVLQQVEHIPKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSASSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPALPVQTTTATTTTAFRAKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNMSGSTHLCLSDRDYEKNGLSGPS

Approximately 53-60 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.