GITR 蛋白, Canine (HEK293, His)

GITR is a type I transmembrane protein. GITR mediates signal transduction through NF-kB and MAPK pathways. Protects T cells from cell death induced by TCR activation. GITR inhibits proliferation and induces apoptosis of Multiple Myeloma (MM) cells in vitro and in vivo. GITR Protein, Canine (HEK293, His) is the recombinant canine-derived GITR protein, expressed by HEK293 , with C-6*His labeled tag.

GITR (Glucocorticoid-induced TNFR-related protein，又称 TNFRSF18 ) 是一种 I 型跨膜蛋白。GITR 刺激效应 T 淋巴细胞增殖，部分逆转 CD4+CD25+ treg 的免疫抑制功能。GITR 在调节性 T 细胞 (Tregs) 和一些活化的免疫细胞上表达，包括效应 T 淋巴细胞、自然杀伤细胞 (NK) 细胞和中性粒细胞。人 GITR 蛋白的氨基酸序列与小鼠 GITR 蛋白同源性较低。GITR 没有任何酶活性，信号通过招募 TRAF 1家族成员，特别是 TRAF1、TRAF2 和 TRAF5 到 GITR 信号复合物中来传播。然后通过 NF-kB 和 MAPK 途径介导信号传导。保护 T 细胞免受 TCR 激活诱导的细胞死亡。GITR 被其配体 GITRL (TNFSF18) 激活。GITR 对小鼠巨噬细胞的 NOS 诱导作用呈时间和剂量依赖性。GITR 在体外和体内抑制多发性骨髓瘤 (Multiple Myeloma, MM) 细胞增殖并诱导细胞凋亡。

Measured by its binding ability in a functional ELISA. Immobilized Canine GITR at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand. The ED₅₀ for this effect is 73.67 ng/mL, corresponding to a specific activity is 1.36×10^4 Unit/mg.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Canine GITR at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand. The ED₅₀ for this effect is 73.67 ng/mL, corresponding to a specific activity is 1.36×10⁴ Unit/mg.

Canine

HEK293

C-6*His

D7F619 (G23-P154)

606971  [NCBI]

GAPSCGPGRLLRGTGTDARCCRPCAPGEAAEKVCPELDCTCVQPGFHCGDPQCKTCKHHTCPPGQEVRPHGNFNFGFECVDCAAGTFSGGQEGRCKPWSDCSQFGYPTTFPGNKTHNAVCSPGLPPTEPRDP

19-25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.