1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins
  4. TNF Receptor Superfamily GITR/CD357
  5. GITR/CD357
  6. GITR 蛋白, Cynomolgus (HEK293, Fc)

GITR 蛋白, Cynomolgus (HEK293, Fc)

目录号: HY-P78716
COA 产品使用指南

GITR 蛋白, Cynomolgus (HEK293, Fc) 是重组的 GITR 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥400 In-stock
10 μg ¥670 In-stock
50 μg ¥1880 In-stock
100 μg ¥3200 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

GITR Protein, Cynomolgus (HEK293, Fc) is the recombinant Rhesus Macaque-derived GITR protein, expressed by HEK293 , with C-hFc labeled tag.

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque GITR at 5 μg/mL (100 μL/well) can bind Biotinylated GITR Ligand. The ED50 for this effect is 0.9219 μg/mL, corresponding to a specific activity is 1.08×10^3 Unit/mg.

  • Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque GITR at 5 μg/mL (100 μL/well) can bind Biotinylated GITR Ligand. The ED50 for this effect is 0.9219 μg/mL, corresponding to a specific activity is 1.08×103 Unit/mg.
种属

Rhesus Macaque

表达系统

HEK293

标签

C-hFc

蛋白编号

XP_005545180.2 (Q20-E155)

基因 ID

102146362

蛋白结构
N-term
GITR (Q20-E155)
Accession # XP_005545180.2
hFc
C-term
中文名
重组猴GITR蛋白
同用名
AITR; GITR; TNFRSF18; CD357
氨基酸序列

QRPTGGPGCGPGRLLLGTGKDARCCRVHPTRCCRDYQSEECCSEWDCVCVQPEFHCGNPCCTTCQHHPCPSGQGVQPQGKFSFGFRCVDCALGTFSRGHDGHCKPWTDCTQFGFLTVFPGNKTHNAVCVPGSPPAE

分子量

Approximately 48 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from sterile 20 mM Tris-HCL, 150 mM NaCL, 100 mM arginine, 150 mM Glycine, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GITR Protein, Cynomolgus (HEK293, Fc)
目录号:
HY-P78716
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