GJA1 蛋白, Human (His)

The GJA1 protein regulates bladder capacity through gap junctions, clusters of transmembrane channels that allow the diffusion of low molecular weight materials between adjacent cells. GJA1 protein also regulates NOV expression and localization and is important for ventricular gap junction communication. GJA1 Protein, Human (His) is the recombinant human-derived GJA1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GJA1 Protein, Human (His) is 150 a.a., with molecular weight of ~22 kDa.

GJA1 蛋白作为间隙连接蛋白，在膀胱容量中发挥调节作用。它由紧密堆积的成对跨膜通道（称为连接子）组成，有助于低分子量材料在相邻细胞之间的扩散。这种蛋白质通过参与钾到耳蜗内淋巴的再循环，在听力生理学中发挥着至关重要的作用。GJA1 作为膀胱功能的负调节剂，增强细胞间的电和化学传递，使膀胱肌肉对胆碱能神经刺激敏感并引起收缩。此外，它可能通过调节 NOV 表达和定位来抑制细胞生长。GJA1 对于心室间隙连接通讯至关重要，它与各种蛋白质相互作用，包括 TJP1、SRC、UBQLN4、SGSM3、CNST、RIC1/CIP150、CSNK1D、NOV 和 TMEM65，从而在细胞通讯和调节中发挥多方面的作用。

Human

E. coli

N-6*His

P17302 (F233-I382)

2697  [NCBI]

FKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Approximately 22 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.