Gliomedin 蛋白, Human (HEK293, N-hFc)

Gliomedin protein is a collagen-rich homotrimer that critically binds to NRCAM and NFASC and plays a key role in establishing and preserving nodes of Ranvier on myelinated axons. It mediates Schwann cell-axon interactions and promotes sodium channel clustering for efficient action potential propagation. Gliomedin Protein, Human (HEK293, N-hFc) is the recombinant human-derived Gliomedin protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Gliomedin Protein, Human (HEK293, N-hFc) is 427 a.a., with molecular weight of 95-112 kDa.

Gliomedin 是一种以胶原样结构域为特征的同源三聚蛋白，作为 NRCAM 和 NFASC/neurofascin 的重要配体，在沿有髓轴突的 Ranvier 节点的建立和保存中发挥着关键作用。其意义在于通过与 NRCAM 和 NFASC 结合来介导雪旺细胞微绒毛和轴突之间的相互作用。朗飞结是有髓轴突上的重要区域，其中包含对动作电位的跳跃传播至关重要的钠通道。格洛梅定通过促进半结点的融合，专门参与发育过程中节点的形成，并且它对于半结点处钠通道的正确聚集是必不可少的。此外，与 NRCAM 合作，Gliomedin 有助于维持 Ranvier 完全成熟节点上的 NFASC 和钠通道簇。此外，它与神经胶质 NRCAM 的相互作用增强了它与轴突 NFASC 的结合亲和力。格里奥梅定的复杂参与强调了其在 Ranvier 节点的结构完整性和功能组织中的重要性。

Human

HEK293

N-hFc

Q6ZMI3-2 (M1-Q427)

342035  [NCBI]

MVDLCNSTKGICLTGPSGPPGPPGAGGLPGHNGLDGQPGPQGPKGEKGANGKRGKMGIPGAAGNPGERGEKGDHGELGLQGNEGPPGQKGEKGDKGDVSNDVLLAGAKGDQGPPGPPGPPGPPGPPGPPGSRRAKGPRQPSMFNGQCPGETCAIPNDDTLVGKADEKASEHHSPQAESMITSIGNPVQVLKVTETFGTWIRESANKSDDRIWVTEHFSGIMVKEFKDQPSLLNGSYTFIHLPYYFHGCGHVVYNNSLYYHKGGSNTLVRFEFGQETSQTLKLENALYFDRKYLFANSKTYFNLAVDEKGLWIIYASSVDGSSILVAQLDERTFSVVQHVNTTYPKSKAGNAFIARGILYVTDTKDMRVTFAFDLLGGKQINANFDLRTSQSVLAMLAYNMRDQHLYSWEDGHLMLYPVQFLSTTLNQ

95-112 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.