GLP1R 蛋白, Human (HEK293, N-His, C-Myc)

The GLP1R protein is a G protein-coupled receptor for glucagon-like peptide 1 (GLP-1), which upon ligand binding activates adenylyl cyclase and increases cAMP levels. This interaction critically regulates insulin secretion, affecting cellular responses and metabolic processes associated with GLP-1 signaling. GLP1R Protein, Human (HEK293, N-His, C-Myc) is the recombinant human-derived GLP1R protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GLP1R Protein, Human (HEK293, N-His, C-Myc) is 122 a.a., with molecular weight of 33 kDa.

GLP1R 蛋白作为胰高血糖素样肽 1 (GLP-1) 的 G 蛋白偶联受体发挥作用，参与配体结合后的信号级联反应，激活腺苷酸环化酶并增加细胞内 cAMP 水平。这种分子相互作用在响应 GLP-1 调节胰岛素分泌方面发挥着至关重要的作用。该受体的激活有助于调节与 GLP-1 信号传导相关的细胞反应和代谢过程。值得注意的是，变构调节剂 NNC0640、PF-06372222 和 MK-0893 对响应 GLP-1 的细胞内 cAMP 水平增加具有抑制作用，为对该信号通路进行药物干预提供了潜在途径。

Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R at 2 μg/mL can bind Anti-GLP1R recombinant antibody, the EC₅₀ is ≤95 ng/mL.

Human

HEK293

C-Myc;N-10*His

P43220 (R24-Y145)

2740  [NCBI]

RPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLY

33 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.