1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GLT25D2 蛋白, Human (sf9, His)

GLT25D2 蛋白, Human (sf9, His)

目录号: HY-P76954
产品使用指南

GLT25D2蛋白是一种β-半乳糖基转移酶,催化β-半乳糖转移到胶原蛋白羟赖氨酸残基上,从而影响胶原蛋白的结构多样性和功能特性。这种糖基化调节胶原蛋白的生化和生物力学特性,影响组织发育、维护和修复。GLT25D2 蛋白, Human (sf9, His) 是重组的 GLT25D2 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

GLT25D2 protein is a β-galactosyltransferase that catalyzes the transfer of β-galactose to collagen hydroxylysine residues, thereby affecting the structural diversity and functional properties of collagen.This glycosylation regulates the biochemical and biomechanical properties of collagen, affecting tissue development, maintenance, and repair.GLT25D2 Protein, Human (sf9, His) is the recombinant human-derived GLT25D2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

GLT25D2 蛋白充当 β-半乳糖基转移酶,专门催化 β-半乳糖转移至胶原蛋白内的羟赖氨酸残基。这种酶活性在胶原蛋白的翻译后修饰中起着至关重要的作用,有助于这种重要的细胞外基质蛋白的结构多样性和功能特性。通过促进羟赖氨酸的糖基化,GLT25D2 参与胶原蛋白生化和生物力学特性的调节。了解 GLT25D2 的作用可以增强我们对胶原蛋白修饰途径的理解,对组织发育、维护和修复具有潜在影响。对 GLT25D2 功能的进一步探索有望增进我们对胶原蛋白生物学的了解,并可能有助于开发针对胶原蛋白相关疾病的治疗策略。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q8IYK4 (M1-S622)

基因 ID
蛋白结构
N-term
GLT25D2 (M1-S622)
Accession # Q8IYK4
His
C-term
同用名
Procollagen galactosyltransferase 2; ColGalT 2; COLGALT2; C1orf17; KIAA0584
分子量

Approximately 68 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GLT25D2 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GLT25D2 Protein, Human (sf9, His)
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