1. 重组蛋白
  2. Viral Proteins
  3. Glycoprotein B/gB 蛋白, CyCMV (HEK293, Fc)

Glycoprotein B/gB 蛋白, CyCMV (HEK293, Fc)

目录号: HY-P77075
产品使用指南

糖蛋白 B (gB) 是一种关键的包膜糖蛋白。gB 在病毒和细胞膜融合中起着关键作用,促进病毒进入宿主细胞。gB 与硫酸肝素蛋白聚糖相互作用,并与 gH/gL 异二聚体形成关联。gB 通过 TLR2 和 MyD88/ TRAF6 依赖性信号通路激活 NF-κBGlycoprotein B/gB 蛋白, CyCMV (HEK293, Fc) 是重组的 糖蛋白 B/gB 蛋白,由 HEK293 表达,带有 C-hFc 标签。Glycoprotein B/gB 蛋白, CyCMV (HEK293, Fc) 全长 453 个氨基酸,分子量约为 ~102.5 KDa。

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描述

Glycoprotein B (gB) is a key envelope glycoprotein. gB plays a key role in the fusion of viruses and cell membranes, facilitating the entry of viruses into host cells. gB interacts with heparin sulfate proteoglycans and is associated with gH/gL heterodimers. gB is activated by TLR2 and MyD88/ TRAF6-dependent signaling pathways NF-κB. Glycoprotein B/gB Protein, CyCMV (HEK293, Fc) is the recombinant Virus-derived Glycoprotein B/gB protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Glycoprotein B/gB Protein, CyCMV (HEK293, Fc) is 453 a.a., with molecular weight of ~102.5 KDa.

研究背景

糖蛋白 B (gB) 是一种关键的包膜糖蛋白,在病毒粒子表面形成独特的尖刺,在宿主细胞表面蛋白聚糖的硫酸肝素部分的初始附着中起着至关重要的作用。这种相互作用是病毒进入过程中的一个基本步骤。此外,gB 在病毒和细胞膜融合中起着关键作用,促进病毒进入宿主细胞。该过程所需的复杂融合机制至少包括 gB 和 gH/gL 异二聚体。除了在进入宿主细胞时的附着和膜融合作用外,gB 还可能在病毒粒子离开时参与病毒粒子包膜与外核膜的融合。作为一种由二硫键连接的三聚体,gB 与硫酸肝素蛋白聚糖相互作用,并与 gH/gL 异二聚体形成关联,进一步强调了其在病毒生命周期中的多面功能。gB 通过 TLR2 和 MyD88/ TRAF6 依赖性信号通路激活 NF-κB

种属

Virus

表达系统

HEK293

标签

C-hFc

蛋白编号

ADV35145 (A228-D680, with cleavage site mutated RHKR to TTQT)

基因 ID

/

蛋白结构
N-term
CyCMV gB (A228-D680, with cleavage site mutated RHKR to TTQT)
Accession # ADV35145
hFc
C-term
同用名
Macaca fascicularis Cytomegalovirus (CyCMV) (strain OT-1) glycoprotein B / GB Protein (Fc)
分子量

Approximately 102.5 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Glycoprotein B/gB Protein, CyCMV (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Glycoprotein B/gB Protein, CyCMV (HEK293, Fc)
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HY-P77075
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