1. 重组蛋白
  2. Viral Proteins
  3. glycoprotein B/gB 蛋白, HHV-7 (Cell-Free, His)

glycoprotein B/gB 蛋白, HHV-7 (Cell-Free, His)

目录号: HY-P702290
产品使用指南

糖蛋白 B (gB) 是一种重要的包膜糖蛋白,可在病毒包膜上形成刺突,并促进与宿主细胞表面硫酸乙酰肝素部分的初始附着。gB 对于病毒进入至关重要,并参与介导病毒和细胞膜之间的融合。glycoprotein B/gB 蛋白, HHV-7 (Cell-Free, His) 是重组的 糖蛋白 B/gB 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。glycoprotein B/gB 蛋白, HHV-7 (Cell-Free, His) 全长 800 个氨基酸,分子量为 93.5 kDa。

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描述

Glycoprotein B (gB) is an important envelope glycoprotein that forms spikes on the viral envelope and facilitates initial attachment to the heparan sulfate moiety on the host cell surface. gB is essential for viral entry and is involved in mediating fusion between the viral and cell membranes. glycoprotein B/gB Protein, HHV-7 (Cell-Free, His) is the recombinant Virus-derived glycoprotein B/gB protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of glycoprotein B/gB Protein, HHV-7 (Cell-Free, His) is 800 a.a., with molecular weight of 93.5 kDa.

研究背景

糖蛋白 B (gB) 蛋白是一种包膜糖蛋白,在病毒包膜表面形成刺突。它对于宿主细胞表面蛋白聚糖上硫酸乙酰肝素部分的初始附着至关重要。此外,gB参与病毒和细胞膜的融合,促进病毒进入宿主细胞。该融合过程由至少由 gB 和异二聚体 gH/gL 组成的融合机制介导。此外,gB 可能在病毒体排出过程中病毒体包膜和外核膜之间的融合中发挥作用。它以通过二硫键连接的同源三聚体形式存在,能够与硫酸乙酰肝素蛋白聚糖结合。此外,gB 与 gH/gL 异二聚体相互作用。

种属

Virus

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P52352 (D23-L822)

基因 ID

/

蛋白结构
N-term
10*His
HHV-7 gB (D23-L822)
Accession # P52352
C-term
同用名
Envelope glycoprotein B
氨基酸序列

DFVMTGHNQHLPFRICSIATGTDLVRFDREVSCASYGSNIKTTEGILIIYKTKIEAHTFSVRTFKKELTFQTTYRDVGTVYFLDRTVTTLPMPIEEVHMVNTEARCLSSISVKRSEEEEYVAYHKDEYVNKTLDLIPLNFKSDTVRRYITTKEPFLRNGPLWFYSTSTSINCIVTDCIAKTKYPFDFFALSTGETVEGSPFYNGINSKTFNEPTEKILFRNNYTMLKTFDDGSKGNFVTLTKMAFLEKGNTIFSWEVQNEESSICLLKHWMTIPHALRAENANSFHFIAQELTASFVTGKSNYTLSDSKYNCINSNYTSILDEIYQTQYNNSHDKNGSYEIFKTEGDLILIWQPLIQRKLTVLENFSNASRKRRKRELETNKDIVYVQLQYLYDTLKDYINTALGKLAEAWCLNQKRTITVLHELSKISPSGIISAVYGKPMSAKLIGDVLAVSKCIEVNQTSVQLHKSMRLTKDSSYDALRCYSRPLLTYSFANSSKETYLGQLGLDNEILLGNHRTEECEQSNTKIFLSGKFAHIFKDYTYVNSSLITEIEALDAFVDLNIDPLENADFTLLELYTKDELSKANVFDLETILREYNSYKSALHHIETKIATVTPTYIGGIDTFFKGLGALGLGLGAVLGVTAGALGDVVNGVFSFLKNPFGGALTILLTLGVIGLVIFLFLRHKRLAQTPIDILFPYTSKSTNSVLQATQSVQAQVKEPLDSSPPYLKTNKDTEPQGDDITHTNEYSQVEALKMLKAIKLLDESYKKAEIAEAKKSQRPSLLERIQYRGYQKLSTEEL

分子量

93.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

glycoprotein B/gB Protein, HHV-7 (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
glycoprotein B/gB Protein, HHV-7 (Cell-Free, His)
目录号:
HY-P702290
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