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  3. Glypican-2/GPC2 蛋白, Mouse (HEK293, His)

Glypican-2/GPC2 蛋白, Mouse (HEK293, His)

目录号: HY-P77957
产品使用指南

Glypican-2 (GPC2) 是一种含有硫酸乙酰肝素的细胞表面蛋白聚糖,可能会影响发育中神经元的运动行为。Glypican-2/GPC2 蛋白, Mouse (HEK293, His) 是重组的 Glypican-2/GPC2 蛋白,由 HEK293 表达,带有 C-His 标签。Glypican-2/GPC2 蛋白, Mouse (HEK293, His) 全长 535 个氨基酸,分子量为 32-35 kDa。

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描述

Glypican-2 (GPC2) is a heparan sulfate-containing cell surface proteoglycan that may influence the motor behavior of developing neurons. Glypican-2/GPC2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Glypican-2/GPC2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Glypican-2/GPC2 Protein, Mouse (HEK293, His) is 535 a.a., with molecular weight of 32-35 kDa.

研究背景

Glypican-2 (GPC2) 蛋白是一种细胞表面蛋白聚糖,其特征在于存在硫酸乙酰肝素。它可能在发育神经元的运动行为中发挥作用。GPC2 通过其硫酸乙酰肝素侧链与多效素 (PTN) 相互作用,促进神经突生长。这种相互作用涉及 PTN 与蛋白多糖硫酸软骨素的结合,导致硫酸软骨素蛋白多糖 (CSPG) 释放蛋白酪氨酸磷酸酶受体 σ (PTPRS),并随后与 GPC2 的硫酸乙酰肝素结合。此外,GPC2 通过其硫酸乙酰肝素链与 Midkine (MDK) 相互作用,诱导 GPC2 聚类。这种相互作用被肝素和硫酸软骨素 E 抑制,并诱导神经元细胞粘附和神经突生长。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q8BKV1 (H22-S556)

基因 ID
蛋白结构
N-term
GPC2 (H22-S556)
Accession # Q8BKV1
His
C-term
同用名
Glypican-2; 2410016G05Rik; Cerebroglycan; GPC2
分子量

32-35 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Glypican-2/GPC2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Glypican-2/GPC2 Protein, Mouse (HEK293, His)
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HY-P77957
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