Glypican-3/GPC3 蛋白, Human (HEK293, His-Myc)

The Glypican-3 (GPC3) protein is a cell surface proteoglycan that plays complex regulatory roles in key signaling pathways critical to developmental processes. Through its GPI anchoring, GPC3 negatively regulates the Hedgehog signaling pathway by competing with the Hedgehog receptor PTC1 for binding to Hedgehog protein, leading to complex internalization and subsequent lysosomal degradation. Glypican-3/GPC3 Protein, Human (HEK293, His-Myc) is the recombinant human-derived Glypican-3/GPC3 protein, expressed by HEK293 , with N-Myc, N-6*His labeled tag and G537R, , , , mutation.

GMP Glypican-3 (GPC3) 蛋白是一种细胞表面蛋白聚糖，在对发育过程至关重要的关键信号通路中发挥着复杂的调节作用。通过其 GPI 锚定，GPC3 通过与刺猬受体 PTC1 竞争与刺猬蛋白的结合，负向调节刺猬信号通路，导致复杂的内化和随后的溶酶体降解。同时，它通过与 Wnt 受体 Frizzled 结合，对经典和非经典 Wnt 信号通路发挥正向调节作用，增强 Frizzled 和 Wnt 配体之间的相互作用。GPC3 与 CD81 结合，减少游离 CD81 与转录抑制因子 HHEX 结合的可用性，导致 HHEX 核易位和转录抑制。此外，GPC3 抑制 DPP4 的二肽基肽酶活性。从功能上讲，GPC3 在肢体模式、骨骼发育、肾分支形态发生和冠状血管发育中发挥着关键作用。它还调节生长因子 BMP2、BMP7 和 FGF7 对肾分支形态发生的影响，并有助于调节原肠胚形成过程中的细胞运动。GPC3 以二硫键形成的异二聚体形式存在，并与多种分子相互作用，包括 DPP4、FGF2、WNT5A、WNT3A、WNT7B、刺猬蛋白 SHH 和 IHH、Wnt 受体 FZD4、FZD7 和 FZD8，显示其在协调发育过程中的关键作用。

Human

HEK293

N-Myc;N-6*His

P51654-1 (Q25-H559, G537R)

2719  [NCBI]

QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPRNSQQATPKDNEISTFHNLGNVH

65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.