GM-CSF 蛋白, Human (His)

GM-CSF Protein, Human is a hematopoietic growth factor and immune modulator.

粒细胞巨噬细胞集落刺激因子（GM-CSF）是由多种细胞类型（包括 T 细胞、巨噬细胞、内皮细胞和成纤维细胞）在接受免疫刺激后产生的。它是一种重要的造血生长因子和免疫调节剂。GM-CSF 对各种循环白细胞的功能活动也有深远的影响。GM-CSF根据其浓度刺激多能祖细胞，最低剂量时巨噬细胞祖细胞的增殖，其次是粒细胞、红细胞、嗜酸性粒细胞、巨核细胞和多能祖细胞。在某些情况下，它还能刺激髓系白血病细胞的分化并控制嗜酸性粒细胞功能^[1][2]。GM-CSF 还增强成熟细胞的功能，例如中性粒细胞。在中性粒细胞中，GM-CSF 增强脱颗粒、氧和氮自由基离子的释放、吞噬作用并抑制细胞凋亡^[3]。某些动物的 GM-CSF 抑制

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED₅₀ for this effect is 26.66-33.71 pg/mL, corresponding to a specific activity is 2.97×10⁷-3.75×10⁷ units/mg.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED₅₀ for this effect is 26.66-33.71 pg/mL, corresponding to a specific activity is 2.97-3.75×10⁷ units/mg.

Human

E. coli

N-6*His

P04141 (A18-E144)

1437  [NCBI]

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Approximately 16 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.