GMP EGF 蛋白, Human

EGF proteins act as potent stimulators of growth in a variety of epidermal and epithelial tissues in both in vivo and in vitro settings, while promoting the proliferation of specific fibroblasts in cell culture. This multifaceted protein also acts as a magnesium stimulating hormone, driving magnesium reabsorption in the renal distal tubule through engagement of EGFR and activation of the magnesium channel TRPM6. GMP EGF Protein, Human is the recombinant human-derived EGF protein, expressed by E. coli , with tag free. The total length of GMP EGF Protein, Human is 53 a.a., with molecular weight of ~9.6 kDa.

GMP EGF 蛋白在体内和体外环境中成为多种表皮和上皮组织生长的有效刺激剂，同时促进细胞培养中特定成纤维细胞的增殖。这种多面蛋白还充当促镁激素，通过 EGFR 的参与和镁通道 TRPM6 的激活驱动肾远曲小管中的镁重吸收。值得注意的是，GMP EGF 蛋白具有在体外诱导池塘蜗牛 Lymnaea stagnalis 运动神经元神经突生长的能力。其分子相互作用包括与 EGFR 结合，从而促进 EGFR 二聚化，以及与 RHBDF2 相互作用，表明参与多种细胞过程。此外，GMP EGF 蛋白与 RHBDF1 结合，可能影响 EGF 在内质网中的保留，并通过内质网相关降解 (ERAD) 调节其降解。

The specific activity is > 1×10⁶ U/mg.

Human

E. coli

Tag Free

P01133 (N971-R1023)

1950  [NCBI]

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Approximately 9.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 200 mM NaCl, pH 8.0.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.