1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HGF & Receptors
  4. HGF
  5. GMP HGF 蛋白, Human (HEK293, His)

GMP HGF 蛋白, Human (HEK293, His)

目录号: HY-P70627G
产品使用指南

HGF 蛋白是一种肝细胞生长因子,参与 MET 和 MAPK 在内的信号通路。HGF 蛋白 由 728 个氨基酸组成,其 α 链含有一个 N 端结构域和 4 个 kringle 结构域,β 有一个丝氨酸蛋白酶结构域。GMP HGF 蛋白, Human (HEK293, His) 是重组的 GMP 级别 HGF 蛋白,由 HEK293 表达,带有 C-6*His 标签。GMP HGF 蛋白, Human (HEK293, His) 全长 697 个氨基酸,分子量分别为 32-38 & 50-65 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK. The HGF Protein consists of 728 amino acids, with an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain. GMP HGF Protein, Human (HEK293, His) is the recombinant human-derived HGF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP HGF Protein, Human (HEK293, His) is 697 a.a., with molecular weight of 32-38 & 50-65 kDa, respectively.

研究背景

Hepatocyte growth factor (HGF) 是肝细胞的有效丝裂原,作为广泛的组织和细胞类型的生长因子。HGF 是Met 受体酪氨酸激酶的激活配体,其活性对正常的细胞发育和器官再生至关重要,但Met 的异常激活与许多类型实体瘤的生长、侵袭和转移有关。HGF 在TMPRSS13 切割和激活后激活MAPK 信号。HGF 是一种由728 个氨基酸组成的双链蛋白,其α 链含有一个 N 端结构域和4 个kringle 结构域,β 有一个丝氨酸蛋白酶结构域。HGF 是纤溶酶原相关生长因子家族的一员,作为一种无活性的单链前体分泌,经过蛋白水解处理形成具有生物活性的二硫化物连接的α/β 异源二聚体。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P14210(Q32-S728)

基因 ID

3082

蛋白结构
N-term
HGF (Q32-S728)
Accession # P14210
6*His
C-term
同用名
Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
氨基酸序列

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

分子量

32-38 &50-65 KDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<10 EU/mg, determined by LAL method.

文件资料

GMP HGF Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
GMP HGF Protein, Human (HEK293, His)
目录号:
HY-P70627G
需求量: