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  3. GNGT1 蛋白, Human (His)

GNGT1 蛋白, Human (His)

目录号: HY-P76369
产品使用指南

GNGT1 蛋白是 G 蛋白家族的成员,在多种跨膜信号系统中至关重要,充当调节剂或转导器。它的 β 链和 γ 链促进 GTP 酶活性,实现 GDP-GTP 交换并介导与效应器的相互作用。G 蛋白具有 α、β 和 γ 三个亚基,协同调节和转导跨细胞膜的信号。GNGT1 蛋白, Human (His) 是重组的 GNGT1 蛋白,由 E. coli 表达,带有 N-His 标签。GNGT1 蛋白, Human (His) 全长 70 个氨基酸,分子量约为 ~9.0 KDa。

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描述

GNGT1 protein, a member of the G protein family, is crucial in diverse transmembrane signaling systems, functioning as a modulator or transducer. Its beta and gamma chains facilitate GTPase activity, enabling GDP-GTP exchange and mediating interactions with effectors. G proteins, with three subunits—alpha, beta, and gamma—collaboratively regulate and transduce signals across cell membranes. GNGT1 Protein, Human (His) is the recombinant human-derived GNGT1 protein, expressed by E. coli , with N-His labeled tag. The total length of GNGT1 Protein, Human (His) is 70 a.a., with molecular weight of ~9.0 KDa.

研究背景

GNGT1 是鸟嘌呤核苷酸结合蛋白(G 蛋白)家族的成员,是多种跨膜信号系统的重要组成部分,充当调节器或转导器。GNGT1 的 β 链和 γ 链在促进 GTP 酶活性、实现 GDP 与 GTP 的交换以及介导 G 蛋白及其效应子之间的相互作用方面发挥着重要作用。G 蛋白的基本结构由三个单元组成:α、β 和 γ,它们协同工作,跨细胞膜调节和转导信号。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P63211 (P2-C71)

基因 ID
蛋白结构
N-term
His
GNGT1 (P2-C71)
Accession # P63211
C-term
同用名
Guanine nucleotide-binding protein G(T) subunit gamma-T1; Transducin gamma chain
分子量

Approximately 9.0 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GNGT1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GNGT1 Protein, Human (His)
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HY-P76369
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