GOT1 蛋白, Human (His)

GOT1 Protein, Human (His), a recombinant human GOT1 produced in E. coli, has a His tag. Calcitonin has an effect on decreasing osteoclast activity and has the potential for hypercalcemia research. Reduction in glutamate concentration in blood is induced through the activity of the blood-resident enzyme glutamate oxaloacetate transaminase 1 (GOT1), which catalyzes the reversible transformation of oxaloacetate and glutamate to aspartate and α-ketoglutarate^[1].

血液谷氨酸清除是一种新颖且有吸引力的保护策略，可减少缺血性脑损伤期间释放的细胞外谷氨酸的兴奋毒性作用。通过施用草酰乙酸激活谷氨酸草酰乙酸转氨酶 1 (GOT1)，已被用于降低血液中谷氨酸浓度^[1]。

Specific activity is 194.189 units/mg, and is defined as the amount of enzyme that converts 1 umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.

Human

E. coli

C-6*His

P17174-1 (A2-Q413)

2805  [NCBI]

APPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

Approximately 45.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 100 mM NaCl, 2 mM DTT, 20% Glycerol, pH 7.5 or PBS, pH 8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. M Pérez-Mato, et al. Human recombinant glutamate oxaloacetate transaminase 1 (GOT1) supplemented with oxaloacetate induces a protective effect after cerebral ischemia. Cell Death Dis. 2014 Jan 9;5(1):e992.  [Content Brief]