1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GOX2015 蛋白, Gluconobacter oxydans (His, Strep)

GOX2015 蛋白, Gluconobacter oxydans (His, Strep)

目录号: HY-P702124
产品使用指南

GOX2015 蛋白表现出显着的氧化 D-葡萄糖和 D-甘露糖的能力,对后者表现出明显的 15 倍偏好。这种催化效率严格依赖于 NADP 的存在,强调了它作为氧化辅助因子的重要作用。GOX2015 蛋白, Gluconobacter oxydans (His, Strep) 是重组的 GOX2015 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。GOX2015 蛋白, Gluconobacter oxydans (His, Strep) 全长 265 个氨基酸。

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描述

The GOX2015 protein showed a significant ability to oxidize D-glucose and D-mannose, showing a clear 15-fold preference for the latter. This catalytic efficiency is strictly dependent on the presence of NADP, emphasizing its important role as an oxidative cofactor. GOX2015 Protein, Gluconobacter oxydans (His, Strep) is the recombinant GOX2015 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of GOX2015 Protein, Gluconobacter oxydans (His, Strep) is 265 a.a., .

研究背景

GOX2015 蛋白表现出氧化 D-葡萄糖和 D-甘露糖的能力,对后者表现出明显的偏好,其催化效率比葡萄糖观察到的催化效率高约 15 倍。这种酶活性严格依赖于 NADP 的存在,强调了它作为氧化过程辅助因子的关键作用。底物特异性和对 NADP 的依赖性强调了 GOX2015 独特的生化特征,揭示了其在细胞代谢途径中的潜在功能意义。

种属

Others

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

Q5FPE5 (P2-S266)

基因 ID

/

蛋白结构
N-term
Strep-6*His
GOX2015 (P2-S266)
Accession # Q5FPE5
C-term
同用名
Glucose 1-dehydrogenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GOX2015 Protein, Gluconobacter oxydans (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GOX2015 Protein, Gluconobacter oxydans (His, Strep)
目录号:
HY-P702124
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