1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GPT 蛋白, Rat (sf9, His)

GPT 蛋白, Rat (sf9, His)

目录号: HY-P75158
产品使用指南

GPT 蛋白通过催化丙氨酸和 2-酮戊二酸之间的可逆氨相互作用产生丙酮酸和谷氨酸产物,在细胞氮代谢中发挥关键作用。这种酶活性是氨基酸代谢动态平衡的重要组成部分,有助于关键代谢物的相互转化。GPT 蛋白, Rat (sf9, His) 是重组的 GPT 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。GPT 蛋白, Rat (sf9, His) 全长 496 个氨基酸,分子量约为 ~48 kDa。

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描述

GPT proteins play a key role in cellular nitrogen metabolism by catalyzing the reversible ammonia interaction between alanine and 2-oxoglutarate to produce pyruvate and glutamate products. This enzyme activity is an integral part of the dynamic balance of amino acid metabolism and contributes to the interconversion of key metabolites. GPT Protein, Rat (sf9, His) is the recombinant rat-derived GPT protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of GPT Protein, Rat (sf9, His) is 496 a.a., with molecular weight of ~48 kDa.

研究背景

GPT 蛋白,也称为丙氨酸转氨酶或 ALT,通过催化丙氨酸和 2-酮戊二酸之间的可逆转氨作用形成丙酮酸和谷氨酸,在细胞氮代谢中发挥着至关重要的作用。这种酶活性是氨基酸相互转化的核心,对于细胞内氮平衡的调节尤其重要。GPT 积极参与肝脏糖异生,有助于从骨骼肌转运的前体合成葡萄糖。GPT 介导的可逆转氨反应是各种代谢途径不可或缺的一部分,强调了其在氨基酸代谢和葡萄糖稳态中的重要性。了解 GPT 的功能有助于深入了解细胞代谢中氮和碳通量的动态调节。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

种属

Rat

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P25409 (M1-S496)

基因 ID
蛋白结构
N-term
GPT (M1-S496)
Accession # P25409
His
C-term
同用名
Alanine aminotransferase 1; ALT1; GPT 1; Aat1
分子量

Approximately 48 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GPT Protein, Rat (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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