Granzyme B/GZMB 蛋白, Mouse (HEK293, His, solution)

Granzyme B/GZMB Protein, found in cytotoxic T-cells and NK-cells, activates caspase-independent pyroptosis and cleaves gasdermin-E (GSDME), inducing target cell death. It also participates in caspase activation cascade, promoting apoptosis and plasma membrane repair during bacterial infection. Granzyme B/GZMB Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived Granzyme B/GZMB protein, expressed by HEK293 , with C-6*His labeled tag.

颗粒酶 B/GZMB 蛋白是一种存在于细胞毒性 T 细胞和 NK 细胞胞质颗粒中的丰富蛋白酶，在各种细胞过程中发挥着至关重要的作用。当通过免疫突触递送至靶细胞时，颗粒酶 B/GZMB 会激活不依赖于半胱天冬酶的细胞焦亡，导致靶细胞死亡。它通过在 Asp 之后裂解并催化 Gasdermin-E (GSDME) 裂解，释放 GSDME 的成孔成分，从而引发细胞焦亡来实现这一目标。颗粒酶 B/GZMB 还参与 caspase 的激活级联，包括 caspase-3、-9 和 -7，它们负责响应细菌感染而进行细胞凋亡执行和质膜修复。

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 2136.752 pmol/min/µg as measured under the described conditions.

Mouse

HEK293

C-6*His

P04187 (G19-S247)

14939  [NCBI]

GEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Approximately 35.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.