Grb2 蛋白, Human (His)

Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag.

Grb2 是一种接头蛋白，在细胞表面生长因子受体与 Ras 信号通路之间建立关键连接方面发挥着关键作用。尽管 Grb2 缺乏与磷酸化表皮生长因子受体 (EGFR) 的直接结合，但它对 EGF 诱导的 RAS 响应元件的反式激活具有抑制作用。值得注意的是，相对于 GRB2，Grb2 作为显性失活蛋白发挥作用，抑制增殖信号并可能引发活跃的程序性细胞死亡。

1.Immobilized Human GRB2 at 2 μg/mL (100 μL/well) can bind Anti-GRB2 Antibody. The ED₅₀ for this effect is 93.39 ng/mL.
2.Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is ≤33.3 ng/mL, corresponding to a specific activity is 3.003×10⁴ units/mg

• 
  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 33.3 ng/mL, corresponding to a specific activity is 3.003×10⁴ units/mg.

Human

E. coli

C-6*His

P62993-1 (M1-V217)

2885  [NCBI]

MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Approximately 25-29 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 8.0 or 50 mM Tris-HCL, 150 mM NaCl, pH 8.0, 8% trehalose, 0.02% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.