Group XV phospholipase A2/Pla2g15, Mouse (His, myc)

Group XV phospholipase A2 (Pla2g15) exhibits dual calcium-dependent phospholipase and O-acyltransferase activities that contribute to glycerophospholipid homeostasis and acyl remodeling in acidic cellular compartments. Group XV phospholipase A2/Pla2g15, Mouse (His, myc) is the recombinant mouse-derived Group XV phospholipase A2/Pla2g15, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Group XV phospholipase A2/Pla2g15, Mouse (His, myc) is 379 a.a., with molecular weight of ~50.5 kDa.

XV 组磷脂酶 A2 (Pla2g15) 表现出双重钙依赖性磷脂酶和 O-酰基转移酶活性，可能有助于甘油磷脂稳态和酸性细胞区室中酰基的重塑。该酶催化磷脂sn-1或sn-2位脂肪酰基的酯键水解，表现出磷脂酶A1和A2的活性，并通过O将其转移到亲脂醇第一个碳上的羟基上。-酰基转移酶活性。Pla2g15 更喜欢脂肪酰基供体，例如磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰丝氨酸，有利于不饱和脂肪酰基的 sn-2 脱酰化而不是 sn-1 脱酰化。此外，它选择性地水解截短的氧化磷脂的 sn-1 脂肪酰基，可能参与氧化应激期间反应性氧化磷脂的解毒。Pla2g15 在肺泡巨噬细胞的磷脂降解中发挥着至关重要的作用，这表明其对肺表面活性物质清除有影响。在中性 pH 值下，它会水解溶血磷脂酰胆碱的 sn-1 脂肪酰基。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-His;C-Myc

Q8VEB4 (A34-P412)

192654  [NCBI]

AQRHPPVVLVPGDLGNQLEAKLDKPKVVHYLCSKKTDSYFTLWLNLELLLPVIIDCWIDNIRLVYNRTSRATQFPDGVDVRVPGFGETFSMEFLDPSKRNVGSYFYTMVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQMYGGPVVLVAHSMGNVYMLYFLQRQPQVWKDKYIHAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNHTWSHEKVFVYTPTTNYTLRDYHRFFRDIGFEDGWFMRQDTEGLVEAMTPPGVELHCLYGTGVPTPNSFYYESFPDRDPKICFGDGDGTVNLESVLQCQAWQSRQEHRVSLQELPGSEHIEMLANATTLAYLKRVLLEP

Approximately 50.5 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<26 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.