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  3. Enzymes & Regulators
  4. GZF1 蛋白, Human (Sf9, His, Strep)

GZF1 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701525
产品使用指南

GZF1 是一种转录抑制因子，通过与具有共有序列 5'-TGCGCN[TG][CA]TATA-3' 的 GZF1 响应元件 (GRE) 结合来调节基因表达。它的作用包括对 VSX2/HOX10 表达的潜在调节，并且它与 NCL 相互作用，表明一种分子关联有助于其转录调节活性。GZF1 蛋白, Human (Sf9, His, Strep) 是重组的 GZF1 蛋白，由 Sf9 insect cells 表达，带有 N-Strep, N-8*His 标签。GZF1 蛋白, Human (Sf9, His, Strep) 全长 710 个氨基酸。

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描述

GZF1, a transcriptional repressor, regulates gene expression by binding to the GZF1 responsive element (GRE) with the consensus sequence 5'-TGCGCN[TG][CA]TATA-3'. Its role includes potential regulation of VSX2/HOX10 expression, and it interacts with NCL, suggesting a molecular association that contributes to its transcriptional modulatory activities. GZF1 Protein, Human (Sf9, His, Strep) is the recombinant human-derived GZF1 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of GZF1 Protein, Human (Sf9, His, Strep) is 710 a.a., .
研究背景

GZF1 是一种转录抑制因子，通过与具有共有序列 5'-TGCGCN[TG][CA]TATA-3' 的 GZF1 响应元件 (GRE) 结合来发挥其调节功能。该蛋白参与 VSX2/HOX10 表达的潜在调节。它与 NCL 的相互作用凸显了有助于其转录调节活性的分子关联。
种属

Human
表达系统

Sf9 insect cells
标签

N-Strep;N-8*His
蛋白编号

Q9H116 (E2-E711)
基因 ID

64412
蛋白结构
N-term
8*His-Strep
GZF1 (E2-E711)
Accession # Q9H116
C-term
同用名
GZF1; GDNF-inducible zinc finger protein 1; Zinc finger and BTB domain-containing protein 23; Zinc finger protein 336
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GZF1 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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